Source: AMICALE DE LA POLICE PHYTOSANITAIRE submitted to
OZONE FUMIGATION TO CONTROL COFFEE LEAF RUST IN GREEN COFFEE BEANS
Sponsoring Institution
Agricultural Research Service/USDA
Project Status
TERMINATED
Funding Source
Reporting Frequency
Annual
Accession No.
0410489
Grant No.
(N/A)
Project No.
5320-43000-014-14S
Proposal No.
(N/A)
Multistate No.
(N/A)
Program Code
(N/A)
Project Start Date
Jan 26, 2007
Project End Date
Jul 31, 2008
Grant Year
(N/A)
Project Director
ARMSTRONG J W
Recipient Organization
AMICALE DE LA POLICE PHYTOSANITAIRE
DEPARTMENT OF PLANT PROTECTION B. P. 100
98713 PAPEETE, TAHITI,null null
Performing Department
(N/A)
Non Technical Summary
(N/A)
Animal Health Component
25%
Research Effort Categories
Basic
25%
Applied
25%
Developmental
50%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
21622321130100%
Knowledge Area
216 - Integrated Pest Management Systems;

Subject Of Investigation
2232 - Coffee;

Field Of Science
1130 - Entomology and acarology;
Goals / Objectives
To determine whether ozone fumigation at 10,000 ppm at -32ml Hg of vacuum for 6 hours will control the uredospores of coffee leaf rust, Hemileia vastatrix. The candidate fumigation schedule was shown to control coffee berry borer, Hypothenemus hampei. If successful in controlling coffee leaf rust uredospores, the ozone fumigation schedule will be recommended as an alternative methyl bromide fumigation for green coffee beans.
Project Methods
Coffee leaves with sporulating bodies will be collected from fields infected with coffee leaf rust near the Plant Protection and Quarantine Laboratory located in Papeete, Tahiti, French Polynesia, and fumigated in equipment developed by and shipped to Tahiti from Tahoe Food Technologies, Sparks, Nevada. Control and fumigated leaves will be placed in distilled water or on potato agar diet to observe for germination. Germination is scored as viable uredospores; non-germination is scored as dead uredospores. (Documents SCA with Amicale de la Police Phytosanitaire).

Progress 01/26/07 to 07/31/08

Outputs
Progress Report Objectives (from AD-416) To determine whether ozone fumigation at 10,000 ppm at -32ml Hg of vacuum for 6 hours will control the uredospores of coffee leaf rust, Hemileia vastatrix. The candidate fumigation schedule was shown to control coffee berry borer, Hypothenemus hampei. If successful in controlling coffee leaf rust uredospores, the ozone fumigation schedule will be recommended as an alternative methyl bromide fumigation for green coffee beans. Approach (from AD-416) Coffee leaves with sporulating bodies will be collected from fields infected with coffee leaf rust near the Plant Protection and Quarantine Laboratory located in Papeete, Tahiti, French Polynesia, and fumigated in equipment developed by and shipped to Tahiti from Tahoe Food Technologies, Sparks, Nevada. Control and fumigated leaves will be placed in distilled water or on potato agar diet to observe for germination. Germination is scored as viable uredospores; non-germination is scored as dead uredospores. (Documents SCA with Amicale de la Police Phytosanitaire) . Significant Activities that Support Special Target Populations Using an experimental ozone fumigation chamber provided by Tahoe Food Technologies, Sparks, NV and under Specific Cooperative Agreement with the Amicale Police Phytosanitaire, Pape¿ete, Tahiti, coffee leaf rust (CLR) urediniospores obtained by collecting coffee leaves with mature CLR lesions were fumigated with 10,000 ppm dry ozone gas under -32 mm Hg of vacuum at 10-12°C for 15.0, 22.5, 30.0, 60.0, and 300.0 min. Average percent survival at each increasing fumigation time was 7.42, 2.70, 0.072, 0, and 0, respectively. Control (untreated) urediniospores had a survival rate of 31.17±4.76 (mean ± SD). Although CLR urediniospores had been expected to tolerate ozone fumigation, the efficacy tests demonstrated that the urediniospores were instead very susceptible, and that the 6 h ozone fumigation time selected as a quarantine treatment for green coffee imported into Hawaii is approximately 5 h longer than needed to ensure quarantine security. The ADODR monitored progress through regular communications with cooperators and through direct supervision of technical staff conducting experiments in Tahiti.

Impacts
(N/A)

Publications


    Progress 10/01/06 to 09/30/07

    Outputs
    Progress Report Objectives (from AD-416) To determine whether ozone fumigation at 10,000 ppm at -32ml Hg of vacuum for 6 hours will control the uredospores of coffee leaf rust, Hemileia vastatrix. The candidate fumigation schedule was shown to control coffee berry borer, Hypothenemus hampei. If successful in controlling coffee leaf rust uredospores, the ozone fumigation schedule will be recommended as an alternative methyl bromide fumigation for green coffee beans. Approach (from AD-416) Coffee leaves with sporulating bodies will be collected from fields infected with coffee leaf rust near the Plant Protection and Quarantine Laboratory located in Papeete, Tahiti, French Polynesia, and fumigated in equipment developed by and shipped to Tahiti from Tahoe Food Technologies, Sparks, Nevada. Control and fumigated leaves will be placed in distilled water or on potato agar diet to observe for germination. Germination is scored as viable uredospores; non-germination is scored as dead uredospores. (Documents SCA with Amicale de la Police Phytosanitaire) . Significant Activities that Support Special Target Populations This report serves to document research conducted under a specific cooperative agreement between the USDA-ARS, U.S. Pacific Basin Agricultural Research Center and Amicale De La Police Phytosanitaire, Pape¿ete, Tahiti, to provide laboratory space and technical assistance to ARS to develop efficacy data on ozone fumigation under vacuum to control coffee leaf rust uredospores. Additional details of research can be found in the report for the parent CRIS 5320-43000-014-00D, Postharvest Treatment of Tropical Commodities for Quarantine Security, Quality Maintenance, and Value Enhancement. A site visit was made in June, 2007, to meet with Dr. Leon Mu of the Department of Plant Protection, a plant pathologist providing laboratory space and technical assistance in obtaining coffee leaf rust uredospores. Infected coffee was found and leaves with spore bodies were brought to the laboratory. A method of obtaining uredospore germination in water was developed and plans were made for completing the ozone fumigation research in August, 2007. The ADODR monitors progress through regular meetings with cooperators, and through direct supervision of technical staff conducting experiments.

    Impacts
    (N/A)

    Publications