Source: PURDUE UNIVERSITY submitted to
AGROCHEMICAL IMPACTS ON HUMAN AND ENVIRONMENTAL HEALTH: MECHANISMS AND MITIGATION (FROM W1045)
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
NEW
Funding Source
Reporting Frequency
Annual
Accession No.
0223866
Grant No.
(N/A)
Project No.
IND011527R1
Proposal No.
(N/A)
Multistate No.
W-2045
Program Code
(N/A)
Project Start Date
Oct 1, 2010
Project End Date
Sep 30, 2015
Grant Year
(N/A)
Project Director
Sepulveda, M. S.
Recipient Organization
PURDUE UNIVERSITY
(N/A)
WEST LAFAYETTE,IN 47907
Performing Department
Forestry & Natural Resources
Non Technical Summary
The results of these studies will provide insights into the effects of low-dose exposure to various agrochemicals on non-target species. State and Federal regulatory agencies may use this information in decisions regarding the registration and use of these and other agrochemicals based on a similar mechanism of toxicity. There is a critical need to develop animal models to indicate the impact of agrochemicals, including endocrine-disrupting compounds, on non-target species. The development of fish models into research and regulatory tools for assessing the impact of an agrochemical on non-target species will be very valuable in protecting fish and wildlife. These models will also be useful to the agrochemical industry in evaluating the toxicity of existing and proposed products.
Animal Health Component
85%
Research Effort Categories
Basic
15%
Applied
85%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
3140810115060%
3050810102020%
3060810104020%
Goals / Objectives
Determine adverse impacts from agrochemical exposure to cells, organisms, and ecosystems. This research will investigate the environmental impact of agrochemical exposure to non-target organisms from the cellular level to the population level.
Project Methods
Researchers at Purdue University (Indiana) will apply genomic, proteomic, and metabolomic tools to better understand the mechanisms of toxicity as well as develop biomarkers of exposure for a range of environmental contaminants. The test models will include a fish (fathead minnow, Pimephales promelas) and invertebrates (Hyalella azteca and Daphnia magna). For the microarrays, the researchers will utilize a recently developed array that is targeted towards early life stages of fathead minnow, as well as a microarray for H. azteca recently developed in collaboration with scientists from Indiana University and that interrogates over 500 K reads. Proteomic analyzes will be conducted using two-dimensional gel electrophoresis coupled with MALDI TOF/TOF mass spectrometry. For the metabolomic analyses, the researchers will use a combination of GC-MS and LC-MS.

Progress 10/01/12 to 09/30/13

Outputs
Target Audience: 1) Teaching undergraduates mostly from FNR but also from other majors (NRES, AGRY, ASCI, VET, EEE) the topics of a) ecotoxicology, b) aquatic animal health, and c) fisheries sampling techniques and fisheries summer practicum 2) Teaching ecotoxicology and aquatic animal health to MS and PhD students 3) Scientific community via invitations to present my work eiher through lectures, seminars or posters 4) General public when my work is highlighted in public media Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? A total of 1 MS defended and 2 PhD students defended in 2013. A total of 1 MS and 5 PhD are currently in the Sepulveda lab. Two undergraduate students conducted research in the Sepulveda lab in 2013. Three undergraduates are currently conducting research in the Sepulveda lab. How have the results been disseminated to communities of interest? Via publications on scientific journals, and non-refereed literature (theses, dissertations, conference proceedings) and presentations at scientific and other meetings. What do you plan to do during the next reporting period to accomplish the goals? Continue to work on the different proposals and continue to publish and mentor students.

Impacts
What was accomplished under these goals? The following are abstracts of the major papers published by the Sepulveda lab during 2013: 1) Silvernanoparticles (Ag NPs) are gaining popularity as bactericidal agents in commercial products; however, the mechanisms of toxicity (MOT) of Ag NPs to other organisms are not fully understood. It is the goal of this research to determine differences in MOT induced by ionic Ag+and Ag NPs in Daphnia magna, by incorporating a battery of traditional and novel methods. Daphnia embryos were exposed to sublethal concentrations of AgNO3and Ag NPs (130-650 ng/L), with uptake of the latter confirmed by confocal reflectance microscopy. Mitochondrial function was non-invasively monitored by measuring proton flux using self-referencing microsensors. Proton flux measurements revealed that while both forms ofsilversignificantly affected proton efflux, the change induced by Ag NPs was greater than that of Ag+. This could be correlated with the effects of Ag NPs on mitochondrial dysfunction, as determined by confocal fluorescence microscopy and JC-1, an indicator of mitochondrial permeability. However, Ag+was more efficient than Ag NPs at displacing Na+within embryonic Daphnia, based on inductively coupled plasma-mass spectroscopy (ICP-MS) analysis. The abnormalities in mitochondrial activity for Ag NP-exposed organisms suggest ananoparticle-specificMOT, distinct from that induced by Ag ions. We propose that the MOT of each form ofsilverare complementary, and can act in synergy to produce a greater toxic response overall. 2) Althoughfatheadminnows (Pimephales promelas) are commonly used as a model fish in endocrine disruption studies, past studies have not characterizedsex-specificbaselineexpressionof genes involved in sex differentiation duringdevelopmentin this species. Using a sex-linked DNA marker to verify gender, we evaluated theexpressionover time of genes involved in sex differentiation (dmrt1, cyp19a, cyp17, star, esr1, ar) in developingfatheadminnows (10-45 days post hatch). Evaluation of these molecular markers in combination with gender identification help us to better understand the mechanisms regulating sex differentiation infatheadminnows and how endocrine-disrupting chemicals may alter these processes. 3)Atrazine, a herbicide commonly applied to agricultural areas and a common contaminant of potable water supplies, is implicated as an endocrine-disrupting chemical (EDC) and potential carcinogen. Studies show that EDCs can cause irreversible changes in tissue formation, decreasedreproductivepotential, obesity, and cancer. The U.S. Environmental Protection Agency considers anatrazineconcentration of ≤ 3 ppb in drinking water safe for consumption. The specific adverse human health effectsassociatedwith adevelopmentalatrazineexposureand the underlying genetic mechanisms of these effects are not well defined. In this study, zebrafish embryos were exposed to a range ofatrazineconcentrations to establish toxicity. Morphological, transcriptomic, and proteinalterationswere then assessed at 72h postfertilizationfollowingdevelopmentalatrazineexposureat 0, 0.3, 3, or 30 ppb. A significant increase in head length was observed in all threeatrazinetreatments. Transcriptomic profiles revealed 21, 62, and 64 genes with altered expression in the 0.3, 3, and 30 ppbatrazinetreatments, respectively. Altered genes wereassociatedwithneuroendocrineandreproductivesystemdevelopment,function, and disease;cell cyclecontrol; andcarcinogenesis. There was a significant overlap (42 genes) between the 3 and 30 ppb differentially expressed gene lists, with two of these genes (CYP17A1 and SAMHD1) present in all threeatrazinetreatments. Increased transcript levels were translated to significant upregulation in protein expression. Overall, this study identifies genetic and molecular targets altered in response to adevelopmentalatrazineexposureto further define the biological pathways and mechanisms of toxicity. 4)Little is known regarding molecular mechanisms involved in sex determination and differentiation in sturgeon species. We addressed this knowledge gap by using next generation pyrosequencing technology to provide transcript libraries and species-specific sequences for mature gonads of shovelnose sturgeon, Scaphirhynchus platorynchus. We then mined these libraries to identify gender-specific transcripts and quantified relative transcript abundance using quantitative real-time polymerase chain reaction (qPCR). We detected a limited number of genes known to play a role in sex differentiation in other species. The sequence for dmrt1 was found only in the testes library. The abundance of dmrt1 differed slightly between the sexes, but the melt curve suggests that there may be different isoforms of dmrt1 in ovaries and testes of shovelnose sturgeon. The transcription factor foxl2 was 5.3-fold greater in ovaries than in testes. Two antagonists to the Wnt cascade, dickkopf-1 (dkk1) and dapper-1 (dact1), were found only in the ovary library. Results from qPCR indicated that dkk1 and dact1 were upregulated 1,819.1- and 207.5-fold, respectively, in ovaries compared with testes. These results suggest that antagonists to the Wnt cascade may play significant roles in sex differentiation and gonadal development in sturgeon and could serve as sex markers in this group of ancient fish. 5)The benthic macroinvertebrate Diporeia spp. have been extirpated from many areas of the Laurentian Great Lakes, but the mechanisms underlying such declines are not fully understood. Diporeia declines coinciding with the invasion of exotic dreissenid mussels (zebra and quagga) have led to the hypothesis that Diporeia declines are a result of decreased food availability from increasing competition with dreissenids for diatoms. There is additional evidence that Diporeia are negatively affected when in close proximity to dreissenids, probably because of exposure to toxins present in the mussels' pseudofeces. Diporeia are also known to be sensitive to anthropogenic contaminants (such as polychlorinated biphenyls [PCBs]) present in Great Lakes sediments. To better understand the physiological responses of Diporeia to diverse stressors, we conducted three 28-d experiments evaluating changes in the metabolomes of Diporeia (1) fed diatoms (Cyclotella meneghiniana) versus starved, (2) exposed (from Lake Michigan and Cayuga Lake) to quagga mussels (Dreissena bugensis), and (3) exposed to sediments contaminated with PCBs. The metabolomes of samples were examined using both two-dimensional gas and liquid chromatography coupled with mass spectrometry. Each stressor elicited a unique metabolome response characterized by enhanced citric acid cycle, fatty acid biosynthesis, and protein metabolism in diatom-fed Diporeia; impaired glycolysis, protein catabolism, and folate metabolism in Diporeia from Lake Michigan irrespective of quagga mussel exposure, suggesting lake-specific adaptation mechanisms; and altered cysteine and phospholipid metabolism during PCB exposure. Subsequent comparisons of these stressor-specific metabolic responses with metabolomes of a feral Diporeia population would help identify stressors affecting Diporeia populations throughout the Great Lakes.

Publications

  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Helen Poynton, Donald Weston, Gary Wellborn, Michael Lydy, Bonnie Blalock, Padrig Tuck, Maria Sepulveda, John Colbourne. Elucidating the mechanism of pesticide resistance in a non-target amphipod, Hyalella azteca: Genetic and Functional Genomic Approaches. 7th Annual Arthropod Genomics Symposium, University of Notre Dame, Notre Dame, IN. June 12-15, 2013.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Jessica K. Leet, Steve Sassman, Jon J. Amberg, Allen W. Olmstead, Linda S. Lee, Gerald T. Ankley, Maria S. Sep�lveda. Environmental hormones and their impacts on sex differentiation in fathead minnows. International Association for Great Lakes Research, Purdue University, West Lafayette, IN June 2-6 2013.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Ahmed AbdelBein, Cecon Mahapatra, and Maria S. Sepulveda. mRNA expression across developmental stages in Japanese medaka (Oryzias latipes). International Association for Great Lakes Research, Purdue University, West Lafayette, IN June 2-6 2013.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Christopher Klinkhamer, Cecon Mahapatra and Maria S. Sep�lveda. Toxicity of the pharmaceuticals cotinine and triclocarban on green algae and diatoms. International Association for Great Lakes Research, Purdue University, West Lafayette, IN June 2-6 2013.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Jenny Zenobio, Brian Sanchez, Laura Archuleta, Jessica Leet, Maria Sep�lveda. PPCPs emerging contaminants on the Baca National Wildlife Refuge, Colorado: Presence and effects on native fish. International Association for Great Lakes Research, Purdue University, West Lafayette, IN June 2-6 2013.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Thijs Bosker, Christopher Perkins, Joseph Griffitt, Maria Sep�lveda. The combined effect of environmental and anthropogenic stressors on fish health. Gulf of Mexico Oil Spill & Ecosystem Science Conference. New Orleans, LA January 21-23, 2013.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Shuai Chen, Garrett J. McKinney, Krista M. Nichols, Maria S. Sep�lveda. In Silico prediction and in vitro validation of Daphnia pulex miRNAs. Interdisciplinary Graduate Research Program Spring Reception, Purdue University, West Lafayette, IN April 1 2013.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: David P. Coulter, Maria S. Sep�lveda, Cary D. Troy, Tomas O. H��k. The role of temperature variability on fish survival, growth, and egg embryo and larval development. American Fisheries Society, Little Rock, AK September 8-12 2013.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Gregory J. Weber, Samuel M. Peterson, Solange S. Lewis, Maria S. Sep�lveda, Jennifer L. Freeman. Transcriptomic profiling of embryos and adult female brain tissue links a developmental origin of atrazine-induced reproductive alterations in zebrafish. 52nd Annual Meeting Society of Toxicology, San Antonio TX March 10-14 2013. AWARDED FIRST PRICE POSTER
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Jenny Zenobio, Brian C. Sanchez, Laura Archuleta, Jessica K. Leet, Maria S. Sep�lveda. Emerging contaminants on the Baca National Wildlife Refuge: presence and effects on native fish. Rocky Mountain SETAC Meeting, Denver CO April 18-19 2013.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Jiejun Gao, Christopher Klinkhamer, Maria S. Sep�lveda, Cecon Mahapatra, Alexander Wei. The effects of silver nanoparticles released from commercial products on zebrafish (Danio rerio) embryos. Society of Toxicology Ohio Valley Chapter, Louisville, KY September 23, 2013.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Shuai Chen, Garrett J. McKinney, Krista M. Nichols, Maria S. Sep�lveda. In Silico prediction and in vitro validation of Daphnia pulex microRNAs. Society of Toxicology Ohio Valley Chapter, Louisville, KY September 23, 2013.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Brian C. Sanchez, Laura Archuleta, Jenny Zenobio, Maria S. Sep�lveda. Emerging contaminants on the Baca National Wildlife Refuge: presence and effects on native fish. 34th Annual Meeting of the Society of Environmental Toxicology and Chemistry, Nashville, TN November 17-21, 2013.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2013 Citation: Ahmed Abdel-moneim, Cecon Mahapatra, Maria S. Sep�lveda. Development of molecular biomarkers for detection of gonadal sex reversal in Japanese medaka after exposure to synthetic estrogens and androgens. Society of Toxicology Ohio Valley Chapter, Louisville, KY September 23, 2013.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Weston DP, Poynton HC, Wellborn GA, Lydy MJ, Blalock BJ, Sep�lveda MS, Colbourne JK. (2013) Variation in sensitivity to pyrethroid insecticides in the amphipod, Hyalella azteca. Proceedings of the National Academy of Sciences 110 (41):16532-16537
  • Type: Journal Articles Status: Awaiting Publication Year Published: 2013 Citation: Leet JK, Schoenfuss HL, Olmstead AW, Amberg JJ!, Ankley GT, Sep�lveda MS. (2013) Sex-specific gonadal and gene expression changes throughout development in fathead minnow. Sexual Development. In Press. Manuscript number SXD0304.
  • Type: Journal Articles Status: Awaiting Publication Year Published: 2013 Citation: Sep�lveda MS, Gonzalez-acu�a D. (2013) Comparison of heavy metals from resident Larus dominicanus and migratory Leucophaeus pipixcan from Talcahuano, Chile. Archivos de Medicina Veterinaria. In Press. Manuscript number: ArchMedVet-Art-491.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Hewson I, Eaglesham J, LaBarre BA, Thompson PD, Watkins J, Rudstam L, H��k TO, Sep�lveda MS. (2013) Investigation of viruses associated with Diporeia spp. from the Laurentian Great Lakes and Owasco Lake, NY as a potential stressor of declining populations. Journal of Great Lakes Research 39:499-506.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Meyer JL, Rogers-Burch S, Leet JK, Villeneuve DL, Ankley GT, Sep�lveda MS. (2013) An evaluation of reproductive physiology in common snapping turtles (Chelydra serpentina) exposed to runoff from a concentrated animal feeding operation. Journal of Wildlife Diseases 49:996-999
  • Type: Journal Articles Status: Awaiting Publication Year Published: 2013 Citation: Soto M, Salas LM, Sep�lveda MS, Kinsella MJ, Mironov S, Gonz�lez-Acu�a D. (2013) First record of parasites from the long-tailed meadowlark Sturnella loyca (Molina 1782) from the Biob�o Region, Chile. Revista Mexicana de Biodiversidad. In Press
  • Type: Journal Articles Status: Awaiting Publication Year Published: 2013 Citation: Amberg JJ, Goforth R, Sep�lveda MS. (2013) Next-generation sequencing reveals sex-specific expression of antagonists to the WNT-cascade in sexually mature shovelnose sturgeon. Sexual Development. In Press. Manuscript number SXD354280.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Weber GJ, Sep�lveda MS, Lewis SS, Peterson SM, Freeman JL. (2013) Transcriptome alterations following developmental atrazine exposure are associated with disruption of neuroendocrine and reproductive system function, cell cycle, and carcinogenesis. Toxicological Sciences 132:458-466
  • Type: Journal Articles Status: Awaiting Publication Year Published: 2013 Citation: Shaui Chen, Garrent J. McKinney, Krista M. Nichols, and Maria S. Sep�lveda. (2013) In silico prediction and in vivo validation of Daphnia pulex microRNAs. PLOS ONE. In Press
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Maity S, Jannasch A, Adamec J, Watkins JM, Nalepa TF, H��k TO, Sep�lveda MS. (2013). Elucidating causes of Diporeia decline in the Great Lakes via metabolomics: Physiological responses after exposure to different stressors. Physiological and Biochemical Zoology 86:213-223
  • Type: Book Chapters Status: Published Year Published: 2013 Citation: Freeman JL, Weber GJ, Sep�lveda MS. (2013) Fishing for microRNAs in Toxicology. In: microRNAs in Toxicology and Medicine. Sahu SC (ed.). John Wiley & Sons Ltd., West Sussex, UK.
  • Type: Journal Articles Status: Under Review Year Published: 2013 Citation: Zenobio JE, Sanchez BC, Archuleta LC, Sep�lveda MS. (2013) Effects and mechanisms of toxicity of triclocarban, DEET and a mixture of other pharmaceuticals on fatehead minnows (Pimephales promelas). Environmental Toxicology and Chemistry.
  • Type: Journal Articles Status: Under Review Year Published: 2013 Citation: Goforth RR, Amberg JJ, Nutile S, Touzinsky K, Bailey EJ, Sep�lveda MS. (2013) Controlling early life history stages of Asian carps using electrical fields and rotenone. Canadian Journal of Fisheries and Aquatic Sciences.
  • Type: Journal Articles Status: Under Review Year Published: 2013 Citation: Coulter DP, Sep�lveda MS, Troy CD, H��k TO. (2013) Habitat quality of aquatic organisms near power plant thermal discharges: Potential exacerbating effects of climate warming. Fisheries Management and Ecology.
  • Type: Journal Articles Status: Awaiting Publication Year Published: 2013 Citation: Stensberg MC, Madangopal R, Yale G, Wei Q, Ochoa-Acu�a H, Wei A, McLamore ES, Rickus J, Porterfield DM, Sep�lveda MS*. (2013) Silver nanoparticle-specific mitotoxicity in Daphnia magna. Nanotoxicology. Manuscript number: TNAN-2013-0115.
  • Type: Journal Articles Status: Awaiting Publication Year Published: 2013 Citation: Sep�lveda MS, Kinsella JM. (2013). Helminth collection and identification from wildlife. Journal of Visualized Experiments. In Press. Manuscript number JoVE 51000.
  • Type: Other Status: Awaiting Publication Year Published: 2013 Citation: Butler LR, Doll JC, Leet JK, Meyer JL, Moraga P, Sep�lveda MS. (2013) Impacts of sewage waste water on feminization and vitellogenin expression in male fathead minnows (Pimephales promelas) from Indiana Waters. Proceedings of the Indiana Academy of Science. In Press.
  • Type: Theses/Dissertations Status: Published Year Published: 2013 Citation: Matthew Stensberg. Development of Real-time Sensing Techniques for Ecotoxicology
  • Type: Theses/Dissertations Status: Published Year Published: 2013 Citation: Gregory J. Weber. Molecular Biomarkers of Exposure to Endocrine Disrupting Herbicides
  • Type: Theses/Dissertations Status: Awaiting Publication Year Published: 2013 Citation: Jenny Zenobio. The Presence of Endocrine Disrupting Compounds, Pharmaceuticals, and Personal Care Products on the Baca National Wildlife Refuge and their Effect on Two Species of Imperiled Fish.


Progress 10/01/11 to 09/30/12

Outputs
OUTPUTS: The goals of this project are to: 1. Identify, develop, and/or validate trace residue analytical methods, immunological procedures, and biomarkers of chemical exposure and effects. 2. Characterize abiotic and biotic reaction mechanisms, transformation rates, and fate of chemicals in agricultural and natural ecosystems. 3. Determine adverse impacts from agrochemical exposure to cells, organisms, and ecosystems. 4. Develop technologies that mitigate adverse human and environmental impacts. PARTICIPANTS: Indiviaduals: Jessica Leet, PhD student, FNR, Purdue University Linda Lee, Professor, Agronomy, Purdue University Tomas Hook, Assistant Professor, Purdue University Partner Organizations: USEPA TARGET AUDIENCES: Formal classroom instruction and laboratory instruction for FNR undergraduate students. Scientific and technical presentations to scientists from academia, government and industry. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
Concentrated animal feeding operation (CAFO) waste is a cost effective fertilizer. In the Midwest, a network of subsurface tile-drains facilitates transport of animal hormones and nutrients from land-applied CAFO waste to adjacent waterways. Our objective was to better understand impacts of land-applied CAFO waste on fish populations and communities. Water hormone concentrations were characterized from study sites. Fish assemblage structure, reproductive condition, and potential endocrine disruption were assessed in selected fish species. Although most CAFO water samples had hormone concentrations < 1 ng/L, peaks in 17&#946;-E2 (32.95 ng/L) and 17&#945;-TB (34.28 ng/L) equivalent concentrations were registered when resident fishes spawn, hatch, and develop. CAFO sites had lower fish species richness, and fishes exhibited lower reproductive condition compared to individuals from the reference site. Fathead minnows (Pimephales promelas) exposed to CAFO ditchwater during early developmental stages exhibited significantly skewed sex ratios towards males. Maximum observed hormone loads were well above the lowest observable effect concentrations for these hormones. Decrease species richness, altered gonadal development, increased growth rates, and skewed sex ratios towards males could be a result of hormone exposure. Further research is therefore needed that investigates potential links between hormone loads and aquatic biotic assemblages.

Publications

  • Baumgarner, B.L., Riley, C.P., Sepulveda, M.S., Brown, P.B., Meyer, J.L., Adamec J., and Sepulveda, M.S. (2012) Increased expression of GAPDH protein is not indicative of nitrosative stress or apoptosis in liver of starved rainbow trout (Oncorhynchus mykiss), Fish Physiology and Biochemistry, 38:379-398.
  • Maity, S., Jannash, A., Adamec, J., Gribskov, M., Nalepa, T.F, Hook TO, Sepulveda MS. (2012 a) Metabolite profiles in starved Diporeia spp. using liquid chromatography-mass spectrometry (LC-MS) based metabolomics, Journal of Crustacean Biology, 32:239-248.
  • Maity. S., Jannasch, A., Adamec, J., Nalepa, T.F., Hook, T.O., and Sepulveda, M.S. (2012 b) Starvation causes disturbance in amino acid and fatty acid metabolism in Diporeia spp., Comparative Biochemistry and Physiology Part B Biochemistry and Molecular Biology, 161:348-355.
  • Moraga. P., Kinsella, J.M., and Sepulveda, M.S. (2012) Helminth parasites of Eastern box turtles (Terrapene carolina carolina) from Southern Indiana, USA, Journal of Helminthology, 86:38-40.
  • Nutile, S., Amberg, J.J., Sepulveda, M.S., and Goforth, R.R. (2012) Effects of multiple electrical field exposures on Cyprinid embryo survival, North American Journal of Fisheries Management, 32:875-879.
  • Ryan, D.J., Sepulveda, M.S., Nalepa, T.F., and Hook, T.O. (2012) Spatial variation in RNA:DNA ratios of Diporeia spp. in the Great Lakes region, Journal of Great Lakes Research, 38:187-195.
  • Sepulveda, M.S., Patrick, H.K., and Sutton, T.M. 2012a) A single se lamprey attack causes acute anemia and mortality in lake sturgeon, Journal of Aquatic Animal Health, 24:91-99.
  • Sepulveda, M.S., Sutton, T.M., Patrick, H.K., and Amberg, J.J. 2012b) Blood-chemistry values for shovelnose and lake sturgeon, Journal of Aquatic Animal Health, 24:135-140.


Progress 10/01/10 to 09/30/11

Outputs
OUTPUTS: Results from our research was disseminated through attendance to scientific national and international meetings (total of 49 meetings which were attended by the PI and/or her graduate and undergraduate students); seminars (7) given by the PI; publications in peer-reviewed journals (16); publications in proceedings (1); book chapters (2); invited lectures (29); and extension events (18). PARTICIPANTS: Nothing significant to report during this reporting period. TARGET AUDIENCES: Undergraduate and graduate students (PhD and MS) General public Federal and state agencies Scientific community, including other researchers PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
These studies have helped in the development of a set of sensitive and robust biomarkers for the quantification of exposure and effects of environmental contaminants on fish and wildlife. The long-term goal of this research is the utilization of these biological responses as early warning signs of problems and for later application in ecological risk assessment evaluations.

Publications

  • Leet J, Gall H, Sepulveda MS. 2011 A review of studies on androgen and estrogen exposure in fish early life stages: effects on gene and hormonal control of sexual differentiation. Journal of Applied Toxicology 31:379-398.
  • Stensberg M, McLamore ES, Porterfield DM, Wei Q, Wei A, Sepulveda MS 2011. Toxicological studies on silver nanoparticles: challenges and opportunities in assessment, monitoring and imaging. Nanomedicine 6:879-898.
  • McLamore ES, Sttensberg M, Sepulveda MS, Zhang W, Banks MK, Porterfield DM. 2011 A self-referencing electrode for real time measurements of ionic silver flux. Sens. Actuat. (B: Chem.) 153:445-452.
  • Johns SM, Denslow ND, Kane MD, Watanabe KH, Orlando EF, Sepulveda MS. 2011 Effects of estrogens and antiestrogens on gene expression of fathead minnow (Pimephales promelas) early life stages. Environmental Toxicology 26:195-206.
  • Ralston-Hooper K, Sanchez BC, Jannash A, Adamec J, Sepulveda MS. 2011 Proteomics in aquatic amphipods: Can it be used to determine mechanisms of toxicity and interspecies responses after exposure to atrazine Environmental Toxicology and Chemistry 30:1197-1203.
  • Sanchez BC, Ralston-Hooper K, Sepulveda MS. 2011 A review of recent proteomic applications in aquatic toxicology. Environmental Toxicology and Chemistry 30:274-282.
  • Li Z, Kroll KJ, Jensen KM, Villeneuve DL, Ankley GT, Brian JV, Sepulveda MS, Orlando EF, Lazorchak JM, Kostich M, Armstrong B, Denslow ND, Watanabe KH. 2011 A computational model of the hypothalamic: pituitary: gonadal axis in female fathead minnows (Pimephales promelas) exposed to 17α-ethynylestradiol and 17β-trenbolone. BMC Systems Biology 5:63. doi: 10.1186/1752-0509-5-63.
  • Ralston-Hooper KJ, Adamec J, Jannash A, Mollenhauer R, Ochoa-Acuna H, Sepulveda MS. 2011 Use of GCxGC/TOF-MS and LC/TOF-MS for metabolomic analysis of Hyalella azteca chronically exposed to atrazine and Its primary metabolite, desethylatrazine. Journal of Applied Toxicology 31:399-410.
  • Carlson-Bremer D, Norton TM, Gilardi KV, Dierenfeld ES, Winn B, Sanders FJ, Cray C, Oliva M, Chen TC, Gibbs SE, Sepulveda MS, Johnson CK. 2010 Health assessment of American oystercatchers (Haematopus palliatus palliatus) in Georgia and South Carolina. Journal of Wildlife Diseases 46:772-780.
  • Sepulveda MS, Stefanavage T, Goforth R. 2010 First record of Polypodium sp. parasitizing eggs from female shovelnose sturgeon from the Wabash River, Indiana. Journal of Aquatic Animal Health 22:36-38.
  • Sanchez BC, Carter BJ, Hammers HR, Sepulveda MS. 2011 Transcriptional response of hepatic largemouth bass (Micropterus salmoides) mRNA upon exposure to environmental contaminants. Journal of Applied Toxicology 31:108-116.
  • Laban G, Nies LF, Turco RF, Bickham JW, Sepulveda MS. 2010 The effects of silver nanoparticles on development of fathead minnow (Pimephales promelas) embryos. Ecotoxicology 19:185-195.
  • Yin-Ming K, Sepulveda MS, Sutton TM, Ochoa-Acuna HG, Muir AM, Miller B, Hua I. 2010 Bioaccumulation and biotransformation of decabromodiphenyl ether in juvenile lake whitefish (Coregonus clupeaformis). Ecotoxicology 19:751-760.
  • Yin-Ming K, Sepulveda MS, Hua I, Ochoa-Acuna HG, Sutton, TM. 2010 Biomagnification and trophic transfer of polybrominated diphenyl ethers (PBDEs) in a food web of Lake Michigan. Ecotoxicology 19:623-634.
  • Sepulveda MS, Ralston-Hooper K, Sanchez BC, Hopf-Jannash A, Baker SD, Diaz N, Adamec J 2010. Use of Proteomics and Metabolomics Techniques in Ecotoxicology. In: Handbook of Systems Toxicology: From Omics Technology to Nanotechnology. Sahu SC and Casciano DA (eds.). John Wiley & Sons Ltd. By Invitation Only.
  • Ralston-Hooper K, Hopf A, Adamec J, Sepulveda MS 2010. The Use of GCxGC/TOF-MS for Metabolomic Analysis of Polar Metabolites. In: Methods in Molecular Biology: Metabolic Profiling. Metz T (ed.). Humana Press. By Invitation Only.
  • McLamore ES, Stensberg M, Yale G, Ochoa-Acuna H, Sepulveda MS, Sun X, Akkus O, Porterfield DM. 2010. An optical imaging technique for monitoring real time changes in morphology within the cell, tissue, or whole organism spatial domain. Proceedings of SPIE-International Society of Optical Engineering, Defense, Security, and Sensing Conference, Orlando, FL. Paper No. 7674-14.