Source: UNIVERSITY OF MISSOURI submitted to
WINTER WHEAT BREEDING PROGRAM
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
TERMINATED
Funding Source
Reporting Frequency
Annual
Accession No.
0211220
Grant No.
(N/A)
Project No.
MO-PSSL0796
Proposal No.
(N/A)
Multistate No.
(N/A)
Program Code
(N/A)
Project Start Date
Sep 1, 2006
Project End Date
Sep 1, 2011
Grant Year
(N/A)
Project Director
McKendry, AN.
Recipient Organization
UNIVERSITY OF MISSOURI
(N/A)
COLUMBIA,MO 65211
Performing Department
Plant Sciences
Non Technical Summary
Fusarium head blight (scab) has caused serious losses in both the yield and quality of wheat in warm and humid regions of the world. The development of scab resistant winter wheat requires identification and genetic characterization of new sources of resistance thereby enabling breeders to put different resistance genes into wheat varieties. This should lead to higher yields and greater economic returns for wheat farmers world-wide.
Animal Health Component
(N/A)
Research Effort Categories
Basic
(N/A)
Applied
(N/A)
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2014020104030%
2021542108030%
2121542108030%
2121542108110%
Goals / Objectives
Objectives of the Missouri Winter Wheat Breeding Program are partitioned into those long term objectives associated with the wheat breeding program per se and short term objectives associated with research within the wheat breeding program. Within the wheat breeding program, our objectives are to: to develop and release varieties of soft red winter wheat and/or enhanced germplasm with improved grain yield and end use quality; to increase the biological efficiency of soft red winter wheat through the identification and introgression of genes for traits, for example disease resistance, that are important to production in Missouri; and to increase the efficiency of breeding for traits associated with enhanced biological efficiency through genetic studies of both a basic and applied nature. Research objectives over the next five years will include: saturating chromosomes 3BS and 5AL in Ernie with markers in an effort to identify markers more closely associated with Fusariumhead blight resistance (FHB) in this cultivar; mapping gene(s) conditioning low toxin (deoxynivalenol) in Ernie and determining whether identified QTL are the same or different from those conditioning type II FHB resistance in this cultivar; investigating the genetics of resistance to FHB in the Missouri soft red winter wheat cultivar, Truman and the germplasm lines Seu Seun 6, Quaderna, and Colorben 4; continuing our efforts to discover novel sources of resistance, such as those found in Ernie, Truman and Bess, through systematic screening of all advanced breeding lines; haplotyping resistant germplasm identified in the Missouri wheat breeding program or through introductions from breeding programs (both national and international); and pyramiding known sources of resistance in Missouri advanced breeding lines through marker assisted selection.
Project Methods
Conventional breeding procedures will be used to develop improved varieties and germplasm. Parental material will include germplasm from the Missouri program, regional wheat nurseries and introductions from wheat breeding programs and repositories globally. Early generation populations will be bulked and advanced through the F2 and F3. Head selections based on highly heritable agronomic traits and agronomic worth will be selected in the F4 and planted as F5 head rows. Selections will be made among F5 families and selected rows will then be bulked for preliminary yield analysis in 6-row, 4.5 x 15 ft. plots. In the first year of testing, preliminary yield nurseries will be unreplicated with locations substituting for replications as seed quantities permit. Promising lines retained for a second year of testing will be arranged in a lattice design with 2 replications and planted over four Missouri locations. Additionally, promising lines will be evaluated for soft wheatmilling and baking quality at the USDA-ARS Soft Wheat Quality Lab in Wooster, OH. Advanced yield tests (years 3-5 of yield testing) will be carried out using a lattice design with three replications over four locations. The most promising lines identified from preliminary yield tests in Missouri will be entered (concurrent with Missouri advanced testing) into the regional nursery system. Advanced lines (F7-F8) will be tested in the 5-State Preliminary and Advanced Soft Red Winter Wheat Nurseries. Genotypes that show potential for release will be tested in the Eastern Soft Red Winter Wheat Nursery. Superior lines will be considered for release after three to five years of testing in the MU breeding program and three years of testing in regional nurseries (concurrent with final years of yield testing in the program). Advanced breeding lines will be systematically screened for FHB resistance in the greenhouse and field environments and assayed for deoxynivalenol levels at Michigan StateUniversity. Genetic analyses of new sources of FHB resistance will be conducted following haplotyping of lines in collaboration with Dr. Gina Brown-Guedira, USDA/ARS Research Geneticist, Department of Crop Science, North Carolina State University where the genetic diversity of FHB alleles will be preliminarily assessed. Only those sources containing potentially novel FHB alleles will be mapped. Mapping populations including recombinant inbred lines and doubled haploid populations will be developed. Phenotyping will be done in both the greenhouse and field with protocols developed and published by the Missouri principle investigator. Simple sequence repeats (SSR), amplified fragment length polymorphisms (AFLP), expressed sequence tags (EST) and other markers as they are developed will be used as necessary for genotyping according to published procedures. Once markers are identified and validated, FHB alleles will be pyramided into Missouri breeding lines using marker assistedselection.

Progress 09/01/06 to 09/01/11

Outputs
Target Audience: This project has as its target audiences both the scientific community and clientele who are wheat growers. Information and accomplishments related to Fusarium head blight objectives were communicated to scientists within the U.S. Wheat and Barley Scab Initiative through annual national meetings and proceedings of those meetings and the broader scientific community through peer-peer reviewed publications of the work. The target audiences for varieties developed within the program include: collaborators in the uniform nursery system including those evaluating the Uniform Eastern and Southern Soft Red Winter Nurseries coordinated by Dr. Harold Bockelman, USDA-ARS at Aberdeen ID and those participating in the 5-State Soft Red Winter Wheat Nurseries grown in Missouri, Illinois, Ohio, Indiana and Kentucky. The primary target groups for varieties developed and released within the program are growers in Missouri and surrounding states for whom varieties are developed. Changes/Problems: Nothing Reported What opportunities for training and professional development has the project provided? During the course of this project, 3 doctoral students have been trained and 12 to 15 undergraduate students have worked on various aspects of the reseach conducted. Doctoral students have attended and presented papers or posters atannual meetings including the National Fusarium Head Blight Forum, the American Society of Agronomy Meetings, and the Plant and Animal Genome Conference where they had the opportunity to interact with some of the best scientists in the field. In addition, one of the projects (the Truman mapping project) provided the opportunity for a doctoral student to work collaboratively on phenotyping with wheat breeders at Purdue (Dr. Herb Ohm) and the University of Kentucky (Dr. David Van Sanford) and as such was actively involved and socializedin the team approach to science. Again, related to their professional development, all three students collaborated on a weekly if not daily basis with Dr. Georgia Davis on the Missouri campus and Dr. Perry Gustafson (USDA-ARS at Missouri) on the molecular aspects of their respective projects. How have the results been disseminated to communities of interest? Scholarly resultshave been disseminated to communities of interest through several mechanisms including: peer-reviewed publications, presentations (both oral and poster)at national meetings including the US National Fusarium Head Blight Forum, American Society of Agronomy Meetings and Plant and Animal Genome Meetings and non-peer reviewed proceedings from these meetings. Germplasm and varieties have been shared with interested breeders though regional cooperative nurseries including the Northern and Preliminary Northern Winter wheat Fusarium Head Blight Nurseries, 5-State Advanced and Preliminary Soft Red Winter Wheat Nurseries, and the Uniform Eastern and or SouthernSoft Red Winter Wheat Nurseries coordinated by the USDA-ARS and the Official Variety Trials conducted by Variety Testing Programsat the University of Missouri and surrounding states. Markers associated with Fusarium Head Blight Resistance have been shared with the Eastern Wheat Genotyping Laboratory located in Raleigh North Carolina. Applied work products (varieties) have been disseminated to growers and beyond through the Foundation Seed Organization of the University of Missouri Agricultural Experiment Stationand the Missouri Crop Improvement Association. What do you plan to do during the next reporting period to accomplish the goals? Nothing Reported

Impacts
What was accomplished under these goals? During the period of this project, two wheat varieties were released and are currently in production in Missouri and surrounding states. Milton, released for its excellent yield, test weight and grain quality was released by the Missouri Agricultural Experiment Station as a public variety with PVP sold under Title V as certified seed. MO 080104 released for its excellent yield potential and test weight, high level of Fusarium head blight (FHB) resistance. It is extremely broad adapted and so was licensed so that it could reach as many growers across the region as possible. Beyond variety release per se, most of the accomplishments from this project have centered on genetic analyses (both molecular and conventional) of native FHB sources of resistance identified within the Missouri breeding program. The value in this type of FHB resistance is that it is in adapted soft red winter wheat backgrounds and therefore accelerates the development and release of FHB resistant varieties. This project has characterized the genetics of FHB resistance in two cultivars that have high levels of native FHB resistance that were released from the Missouri breeding program; Ernie, and Truman. A recombinant inbred line (Ril) population constructed from the cross Ernie/MO 94-317 was evaluated in greenhouse and field FHB nurseries. Over several studies, phenotypic data were collected on greenhouse severity, field incidence, severity, FHB index (incidence x severity), Fusarium damaged kernels and deoxynivalenol (DON) content of the infected grain. Genotyping was done using SSR markers and QTL analyses were run. Four QTL on chromosomes 2B (Xgwm276b), 3B (Xgwm285), 4BL (Xgwm495), and 5A (Xbarc165) were associated with greenhouse severity. Results were published by Liu et al. in 2007 in TAG. Abate et al. (Crop Science 2008) later identified 4 QTL on chromosomes 2B (Xgwm276b), 2BSc (Xgwm285), 4BL (Xgwm495), and 5AS (Xbarc56) associated with DON content of the grain and 4 QTL on chromosomes 2B (Xgwm271), 3BSc (Xgwm285), 4BL (Xgwm495), and 5AS (Xbarc165) that were associated with Fusarium damaged kernels (FDK). Two of the four QTL for FDK were in common with those for DON and all of the QTL for DON were co-localized with those associated with greenhouse severity. The population was later phenotyped under field conditions in mist-irrigated, inoculated nurseries at Virginia and Missouri to map resistance for field traits including FHB incidence. The population was also fine-mapped in an effort to identify new, more closely-linked markers associated with traits previously mapped including greenhouse severity, DON and FDK. Thirty-nine new markers were mapped onto chromosomes 2BS, 2D, 3BL, 4BS, 4DS, and 5A and 3 new QTL on 4BS, 4DS, and 5AL were identified that were associated with incidence, severity, and FHBI. A forth QTL on 2DS associated with Ppd-D1 was also detected for severity and index. New QTL were also identified for DON (on 4DS and 6AL) and for FDK (on 4BS and 4DS). QTL on 4DS and 4BS were common with those for components of field incidence and severity. The marker interval for the 4BS QTL was Rht-B1 – Xgwm513 while that on 4DS either included Rht-D1 or had Rht-D1 at the QTL peak. Based on these results, the FHB resistance in Ernie appears to differ from that in other widely used sources of resistance including Sumai 3 and therefore should be a valuable additional resource for marker-assisted gene pyramiding of FHB resistance genes. The genetics of resistance has also been studied in the soft red winter wheat Truman through a large diallel experiment and through genetic mapping using SSR, DArT, and SNP markers in a recombinant inbred line population developed from the cross Truman/MO 94-317. The diallel study suggested a partial dominance component of greenhouse severity but more importantly suggested that epistasis was an important component of the resistance of many widely-used sources which can complicate their use as parents in breeding programs. Epistasis, however, did not appear to be as predominant in Truman, explaining anecdotal evidence from the Missouri breeding program and others that the resistance in Truman is highly penetrant. Across years and locations of phenotyping, four QTL on chromosomes 1BSc, 2BL, 2DS and 3BSc were associated with greenhouse severity; three QTL on 2ASc, 2DS and 3DS were associated with incidence; 2 QTL on 2DS, and 3BSC were associated with severity while 3 QTL on chromosomes 2ASc, 2DS and 3BSc were associated with FHBI. For seed traits, two QTL were identified on 2ASc and 2DS for both FDK and DON while additional QTL on 3BLc (FDK) and 3BSc (DON) were identified that were not common across the seed quality traits. A second study involving 1216 mapped SNPs and 145 SSRs identified QTL in similar regions for most traits examined. Common across greenhouse and field traits was a QTL on 2D that accounted for 16.9 to 19.3% of the variation in disease incidence, severity and FHBI and 30% of the phenotypic variation in DON. This is the largest known effect reported for FHB resistance developed from U.S. sources of wheat (native sources) and should be very useful for marker-assisted-selection because it is common across all important components of FHB resistance and appears to be novel. Four manuscripts from this project are in preparation for publication in Euphytica and Crop Science. Doubled haploid mapping populations have been developed and increased for resistant sources from Italy (Colorben 4, Quaderna) and South Korea (Seu Suen 6). We anticipate mapping FHB resistance in these populations in 2014 along with a RIL population developed from the cross Bess (which is an early maturing full sib of Truman)/MO 94-317. The Bess population will be used to validate the markers identified in the Truman population. Screening of all breeding germplasm for FHB resistance has identified a potentially new source of resistance in MO 080104 which is comparable to the resistance level in Truman but differs from Truman by descent. It is remarkable in that it not only has excellent levels of FHB resistance including low DON but also has extremely good yield potential across the entire soft red winter wheat production area. MO 080104 has been licensed but also serves as a check in the Uniform Eastern Soft Red Winter Wheat Nursery coordinated by Harold Bockelman, USDA-ARS.

Publications

  • Type: Conference Papers and Presentations Status: Published Year Published: 2008 Citation: Liu, S., C. Griffey, A.L. McKendry, and M. Hall. 2008. Saturation mapping of scab resistance QTL in Ernie and application to marker-assisted breeding. In: Canty, S.M., E. Walton, A. Clark, D. Ellis, J. Mundell, and D.A. Van Sanford (Eds.), Proceedings of the National Fusarium Head Blight Forum; (Pp. 180) Dec 2-4; Indianapolis, IN.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2008 Citation: Liu, S., C.A. Griffey, J. Chen, A.L. McKendry, C.H. Sneller, J.P. Murphy, and G. Brown-Guedira. 2008. Breeding and genomics of Fusarium head blight resistance in soft red winter wheat: research progress and ongoing projects. American Society of Agronomy Annual Meetings; 5-9 October, 2008; Houston, TX.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2007 Citation: Liu, S., C.A. Griffey, A.L. McKendry, and M.D. Hall. 2007. Meta-analyses of QTL associated with Fusarium head blight resistance. In: Canty, S.M., Clark, A., Ellis, D., and Van Sanford D. (Eds) Proceedings of the National Fusarium Head Blight Forum. (Pp.195-200). 2-4 December 2007; Westin Crown Center, Kansas City, MO.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2007 Citation: McKendry, A.L. 2007. Breeding for FHB resistance in winter wheat: What's ahead? In: Canty, S.M., Clark, A., Ellis, D., and Van Sanford D. (Eds.) Proceedings of the National Fusarium Head Blight Forum. (Pp. 204). 2-4 December 2007; Westin Crown Center, Kansas City, MO.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2007 Citation: Abate, Z.A., D.N. Tague, and A.L. McKendry 2007. Diallel analysis of Fusarium head blight resistance in genetically diverse winter wheat germplasm. American Society of Agronomy Annual Meetings; New Orleans, LA. Nov. 4-8, 2007.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2007 Citation: F.E. Dowell, E.B. Maghirang, P.S. Baenziger, A.L. McKendry, E.A. Milus, P. Horevaj, S.N. Wegulo, J.A. Breathnach. 2007. Applying single kernel sorting technology to developing scab resistant lines. In: Canty, S.M., Clark, A., Ellis, D., and Van Sanford D. (Eds) Proceedings of the National Fusarium Head Blight Forum. 2-4 December 2007; Westin Crown Center, Kansas City, MO.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2006 Citation: Abate, Z.A., S. Liu, and A.L. McKendry. 2006. QTL associated with low deoxynivalenol and kernel quality retention in the Fusarium head blight resistant cultivar Ernie. In: Proceedings of the 2006 National Fusarium Head Blight Forum, Research Triangle Park, North Carolina. Dec 10-12, 2006. p 78-81.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2006 Citation: Abate, Z.A., D.N. Tague, and A.L. McKendry 2006. Diallel analysis of Fusarium head blight resistance in genetically diverse winter wheat germplasm. In: Proceedings of the 2006 National Fusarium Head Blight Forum, (Pp. 73-77)Research Triangle Park, North Carolina. Dec 10-12, 2006.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2012 Citation: Sneller, C.H., A. Cabrera, P. Paul, D. Van Sanford, A. Clark, A. McKendry, F. Kolb, H. Ohm, R. Freed, M. E. Sorrells. 2012. Phenotypic analysis of a soft wheat population that will be used for association analysis and genomic selection. In: S. Canty, A. Clark, A. Anderson-Scully, and D. Van Sanford (Eds). Proceedings of the 2012 National Fusarium Head Blight Forum (Pp. 99) East Lansing, MI/Lexington, KY: US Wheat and Barley Scab Initiative.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2011 Citation: Islam, Md. S., G. Brown-Guedira, H.Ohm, D. Van Sanford, A.L. McKendry. 2011. QTL associated with Fusarium head blight incidence and severity in Truman soft red winter wheat. In: S. Canty, A. Clark, A. Anderson-Scully, and D. Van Sanford (Eds). Proceedings of the 2011 National Fusarium Head Blight Forum (Pp. 27). East Lansing, MI/Lexington KY: U.S. Wheat & Barley Scab Initiative.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2011 Citation: Islam, Md. S., G. Brown-Guedira, H.Ohm, D. Van Sanford, Y. Dong, A.L. McKendry. 2011. QTL associated with kernel quality retention and DON in Truman soft red winter wheat. In: S. Canty, A. Clark, A. Anderson-Scully, and D. Van Sanford (Eds). Proceedings of the 2011 National Fusarium Head Blight Forum (Pp. 28). East Lansing, MI/Lexington KY: U.S. Wheat & Barley Scab Initiative.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2011 Citation: Islam, Md. S., G. Brown-Guedira, A.L. McKendry. 2011. QTL associated with type II Fusarium head blight resistance in Truman soft red winter wheat. In: S. Canty, A. Clark, A. Anderson-Scully, and D. Van Sanford (Eds). Proceedings of the 2011 National Fusarium Head Blight Forum (Pp. 29). East Lansing, MI/Lexington KY: U.S. Wheat & Barley Scab Initiative.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2011 Citation: Liu, S.Y., C.A. Griffey, M.D. Hall, A.L. McKendry, J. Chen, G. Brown-Guedira, D. Van Sanford, and D.G. Schmale. 2011. Mapping Fusarium head blight resistance in wheat cultivars Ernie and Massey. In: S. Canty, A. Clark, A. Anderson-Scully, and D. Van Sanford (Eds). Proceedings of the 2011 National Fusarium Head Blight Forum (Pp. 34). East Lansing, MI/Lexington KY: U.S. Wheat & Barley Scab Initiative.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2011 Citation: Wright, E., C. Griffey, S. Malla, D. Van Sanford, S. Harrison, J.P. Murphy, J. Costa, G. Milus, J. Johnson, A.L. McKendry, D. Schmale, N. McMaster. 2011. Family based mapping of Fusarium head blight resistance in soft wheat cultivars, Roane and Jamestown. In: S. Canty, A. Clark, A. Anderson-Scully, and D. Van Sanford (Eds). Proceedings of the 2011 National Fusarium Head Blight Forum (Pp. 60). East Lansing, MI/Lexington KY: U.S. Wheat & Barley Scab Initiative.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2009 Citation: Rottinghaus, G.E. B.K. Tacke, T.J. Evans, M.S. Monstrom, L.E. Sweets, and A.L. McKendry. 2009. Fusarium mycotoxin concentrations in straw, chaff and grain of soft red winter wheat expressing a range of resistance to Fusarium head blight. In: Canty, S.M. A. Clark, J. Mundell, E. Walton, D. Ellis, and D. Van Sanford (Eds.) Proceedings of the National Fusarium Head Blight Forum (Pp. 10) 2009 Dec 7-9; Orlando, FL. Lexington KY: University of Kentucky.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2009 Citation: Liu, S. C.A. Griffey, A.L. McKendry, M.D. Hall. 2009. Saturation mapping of scab resistance QTL in Ernie and identification of diagnostic markers for breeding scab resistance. In: Canty, S.M. A. Clark, J. Mundell, E. Walton, D. Ellis, and D. Van Sanford (Eds.) Proceedings of the National Fusarium Head Blight Forum. (Pp. 133) 2009 Dec 7-9; Orlando, FL. Lexington KY: University of Kentucky.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2009 Citation: Liu, S., M.D. Hall, C.A. Griffey, A.L. McKendry, J. Chen, W.S. Brooks, G. Brown-Guedira, and D. Van Sanford. 2009. Saturation mapping QTL for scab resistance in the Virginia wheat cultivar Massey. In: Canty, S.M. A. Clark, J. Mundell, E. Walton, D. Ellis, and D. Van Sanford (Eds.) Proceedings of the National Fusarium Head Blight Forum. (Pp. 134) 2009 Dec 7-9; Orlando, FL. Lexington KY: University of Kentucky.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2009 Citation: Liu, S., C.A. Griffey, A.L. McKendry, J. Chen, M.D. Hall, G. Brown-Guedira. 2009. Marker saturation of QTL for scab resistance in native sources and its application in marker-assisted breeding. Plant and Animal Genome Meeting, San Diego CA.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2008 Citation: Abate, Z., S. Liu, A.L. McKendry. 2008. Validation of QTL associated with Fusarium head blight resistance in the soft red winter wheat, 'Ernie'. In: Canty, S.M., E. Walton, A. Clark, D. Ellis, J. Mundell, and D.A. Van Sanford (Eds.), Proceedings of the National Fusarium Head Blight Forum; Dec 2-4; Indianapolis, IN. pp 125-128.
  • Type: Journal Articles Status: Published Year Published: 2008 Citation: Liu, S., M.D. Hall, C.A. Griffey, A.L. McKendry, J. Chen, and D.A. Van Sanford. 2008. Mapping QTL for scab resistance in the Virginia wheat cultivar Massey. In: Canty, S.M., E. Walton, A. Clark, D. Ellis, J. Mundell, and D.A. Van Sanford (Eds.), Proceedings of the National Fusarium Head Blight Forum; (Pp. 176-177) Dec 2-4; Indianapolis, IN.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2010 Citation: Shoots, J., Guttieri, M., Kolb, F., Lewis, J., A.L. McKendry, H. Ohm, C. Sneller, M.E. Sorrells, E. Souza, D. Van Sanford, J. Costa, C. Griffey, S. Harrison, J. Johnson, and P. Murphy. 2010.Development and Distribution of Male-Sterile Facilitated Recurrent Selection Populations In: S. Canty, A. Clark, A. Anderson-Scully, D. Ellis and D. Van Sanford (Eds). Proceedings of the National Fusarium Head Blight Forum. (Pp.145) 2010 Dec 7-9; Milwaukee, WI.
  • Type: Journal Articles Status: Published Year Published: 2013 Citation: Liu, S. C.A. Griffey, M.D. Hall, A.L. McKendry, J. Chen, W.S. Brooks, G Brown-Guedira, D. Van Sanford, D.G Schmale. 2013. Molecular characterization of field resistance to Fusarium head blight in two US soft red winter wheat cultivars. Theor Appl Genet. DOI 10.1007//s00122-013-2149-y
  • Type: Journal Articles Status: Published Year Published: 2010 Citation: Abate, Z.A., and A.L. McKendry. 2010. Diallel analyses of Fusarium head blight resistance in genetically diverse winter wheat germplasm. Euphytica 175:409-421.
  • Type: Journal Articles Status: Published Year Published: 2009 Citation: Liu, S., M.D. Hall, C.A. Griffey, A.L. McKendry. 2009. Meta-analysis of QTL associated with Fusarium head blight resistance in wheat. Crop Sci. 49:1955-1968.
  • Type: Journal Articles Status: Published Year Published: 2008 Citation: Abate, Z. A., S. Liu, and A.L. McKendry. 2008. Quantitative trait loci associated with deoxynivalenol content and kernel quality in the soft red winter wheat, 'Ernie'. Crop Science 48:1408-1418.
  • Type: Journal Articles Status: Other Year Published: 2013 Citation: Islam, Md. S., G. Brown-Guedira, H.Ohm, D. Van Sanford, A.L. McKendry. 2012. QTL associated with Fusarium head blight incidence and severity in Truman soft red winter wheat. Euphytica. (in preparation).
  • Type: Journal Articles Status: Other Year Published: 2013 Citation: Islam, Md. S., G. Brown-Guedira, H.Ohm, D. Van Sanford, Y. Dong, A.L. McKendry. 2012. QTL associated with kernel quality retention and DON in Truman soft red winter wheat. Euphytica. (in preparation).
  • Type: Conference Papers and Presentations Status: Published Year Published: 2008 Citation: Brown-Guedira, G., C. Griffey, F. Kolb, A.L. McKendry, J.P. Murphy, D.A. Van Sanford. 2008. Breeding FHB-resistant soft red winter wheat: progress and prospects. Cer. Res. Comm. 36 Supplement B. 31-35. (Proceedings of the 3rd International Symposium on Fusarium Head Blight; Szeged, Hungary; 1-5 September 2008.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2008 Citation: McKendry, A.L. 2008. Native resistance: an essential building block for accelerating the development of scab resistant soft red winter wheat. Cer. Res. Comm. 36: 135-137. (Proceedings of the 3rd International Symposium on Fusarium Head Blight; Szeged, Hungary; 1-5 September 2008.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2012 Citation: Van Sanford, D., F. Kolb, A. McKendry, H. Ohm, C. Sneller, M. Sorrells, G. Brown-Guedira, J. Lewis, R. Freed, L. Siler. 2012. Coordinated evaluation and utilization of marker assisted selection for FHB resistance. In: S. Canty, A. Clark, A. Anderson-Scully, and D. Van Sanford (Eds). Proceedings of the 2012 National Fusarium Head Blight Forum (Pp.107) East Lansing, MI/Lexington, KY: US Wheat and Barley Scab Initiative
  • Type: Conference Papers and Presentations Status: Published Year Published: 2012 Citation: Wright, E., C. Griffey, S. Malla, D. Van Sanford, A. McKendry, D. Schmale III, A. Clark, and N. McMaster. 2012. Mapping FHB resistance in SRW wheat cultivar Jamestown. In: S. Canty, A. Clark, A. Anderson-Scully, and D. Van Sanford (Eds). Proceedings of the 2012 National Fusarium Head Blight Forum (Pp. 108) East Lansing, MI/Lexington, KY: US Wheat and Barley Scab Initiative.
  • Type: Journal Articles Status: Published Year Published: 2007 Citation: McKendry, A.L., D.N. Tague, R.L. Wright, J.A. Tremain. 2007. Registration of Bess wheat. Crop Science Journal of Plant Registrations 1:21-23
  • Type: Journal Articles Status: Published Year Published: 2007 Citation: S. Liu, Z. A. Abate, H. Lu, T. Musket, G.L. Davis, and A.L. McKendry. 2007. QTL associated with Fusarium head blight resistance in the soft red winter wheat Ernie. Theor. Appl. Genet. 115:417-427
  • Type: Conference Papers and Presentations Status: Published Year Published: 2012 Citation: McKendry, A.L., Md. S. Islam. 2012. Is the Fusarium head blight resistance in Truman soft red winter wheat novel? In: S. Canty, A. Clark, A. Anderson-Scully, and D. Van Sanford (Eds). Proceedings of the 2012 National Fusarium Head Blight Forum (Pp. 68) East Lansing, MI/Lexington, KY: US Wheat and Barley Scab Initiative. Abstract of Invited Presentation.
  • Type: Conference Papers and Presentations Status: Published Year Published: 2012 Citation: Maloney, P.V., S. Petersen, R.A Navarro, D. Marshall, A.L. McKendry, J.M. Costa, and J.P. Murphy. 2012. Comparison of visual and digital image analysis methods for estimation of Fusarium damaged kernels in wheat. In: S. Canty, A. Clark, A. Anderson-Scully, and D. Van Sanford (Eds). Proceedings of the 2012 National Fusarium Head Blight Forum (Pp. 66) East Lansing, MI/Lexington, KY: US Wheat and Barley Scab Initiative.


Progress 01/01/09 to 12/31/09

Outputs
OUTPUTS: The major objective of the Wheat Breeding Project is to develop varieties of soft red winter wheat and/or enhanced germplasm with improved grain yield, quality and disease resistance. In 2009, a new soft red winter wheat variety, Milton, was released from the breeding program. Milton, tested as MO 011126, was released because of its excellent yield and test weight potential, superior milling and balking quality, resistance to disease prevalent diseases and broad adaptation across the soft red winter wheat production area. Milton carries the Rht2 dwarfing gene and is photoperiod insensitive based on the RHT-D1 and Ppd markers, respectively. Although Milton is considered moderately susceptible to Fusarium head blight, (FHB) it appears to retain good kernel quality in the presence of FHB symptoms in the head. We continue to focus on the identification and genetic analyses of new sources of resistance to FHB. Ongoing work includes the genetic characterization of resistance to FHB in the Missouri cultivar Truman which has excellent levels of types I (low incidence) and II (low severity) resistances and their product (FHB index) as well as low deoxynivalenol (DON) content and low Fusarium damaged kernels (FDK) under heavy disease pressure. A set of 240 recombinant inbred lines, developed from the cross of Truman with MO 94-317 (susceptible to FHB) is being used to map these four components of resistance. A multi-state collaborative approach involving the University of Missouri (lead institution), Purdue University and the University of Kentucky is being used for phenotyping while genotyping is being done at Missouri. In collaboration with Dr. Shuyu Liu at Virginia Tech, we are identifying QTL associated with FHB resistance in the Virginia cultivar Massey and further saturating the genome in an effort to identify better diagnostic markers in the cultivar Ernie. In collaboration with Dr. George Rottinghaus of the Veterinary Medical Diagnostic Laboratory at the University of Missouri we evaluated concentrations of both deoxynivalenol (DON) and zearalenone (ZEA) in the straw, chaff and grain of 60 wheat cultivars varying in FHB resistance. Because swine are sensitive to concentrations of DON and ZEA as low as 1ppm, these mycotoxins if present are particularly problematic when wheat straw is used for bedding in less than optimal production settings. Similarly, where straw is used as a source of roughage for cattle in total mixed rations, concentrations of mycotoxins, to which ruminants are fairly resistant, might be found at clinically relevant high concentrations in the straw. We found alarmingly high levels of zearalenone in the straw of many of the resistant varieties evaluated. The average concentration of ZEA across all varieties was 55 ppm, where sensitivity is seen at 1ppm. The data indicated that mycotoxin concentration in the straw could not be reliably be predicted by the resistance level of the cultivar. These results were troubling and a more rigorous experiment will be run in 2010 to verify these findings. PARTICIPANTS: Principle Investigator: Dr. Anne L. McKendry Wheat Breeding Staff at the University of Missouri: David Tague, Senior Research Specialist; Brendan Craughwell, Research Specialist; Julie Solomon, Senior Research Laboratory Technician.Doctoral Students: Zewdie Abate, Md. Sariful Islam. Collaborators outside the University of Missouri:Dr. Shuyu Liu, Research Scientist Virginia Tech University, Dr. Ed Souza, USDA-ARS Soft Wheat Quality Laboratory, Wooster, OH, Dr. David Van Sanford, University of Kentucky,Dr. Herb Ohm, Purdue University, Scientists within the US Wheat and Barley Scab Initiative TARGET AUDIENCES: Scientists including wheat breeders and plant pathologists working on Fusarium head blight resistance. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
This research will lead to the development and release to Missouri growers, of adapted varieties of soft red winter wheat that have higher yield potential, excellent test weight, good end use quality and resistance to pathogens relevant in the Missouri wheat growing areas. It will also lead to increased breeding efficiency that should accelerate the development of these improved wheat varieties thus enhancing economic return for wheat growers both in Missouri and in the surrounding states. Research associated with the genetic characterization of novel sources of Fusarium head blight resistance will accelerate the development of FHB resistant varieties for Missouri and the surrounding areas. The release of these varieties will strengthen the wheat-based economy of the state and where DON levels in the harvested grain are reduced, they will improve the safety of the wheat-based food supply.

Publications

  • Abate, Z. A., S.Liu, and A.L. McKendry. 2008. Quantitative Trait Loci Associated with Deoxynivalenol Content and Kernel Quality in the Soft Red Winter Wheat, 'Ernie'. Crop Science 48:1408-1418.
  • Brown-Guedira, G., C. Griffey, F. Kolb, A.L. McKendry, J.P. Murphy, D.A. Van Sanford. 2008. Breeding FHB-resistant soft red winter wheat: progress and prospects. Cer. Res. Comm. 36 Supplement B. 31-35. (Proceedings of the 3rd International Symposium on Fusarium Head Blight; Szeged, Hungary; 1-5 September 2008.)
  • McKendry, A.L. 2008. Native resistance: an essential building block for accelerating the development of scab resistant soft red winter wheat. Cer. Res. Comm. 36 Supplement B. 135-137. (Proceedings of the 3rd International Symposium on Fusarium Head Blight; Szeged, Hungary; 1-5 September 2008.)
  • Abate, Z., S. Liu, A.L. McKendry. 2008. Validation of QTL associated with Fusarium head blight resistance in the soft red winter wheat, 'Ernie'. In: Canty, S.M., E. Walton, A. Clark, D. Ellis, J. Mundell, and D.A. Van Sanford (Eds.), Proceedings of the National Fusarium Head Blight Forum; 2008 Dec 2-4; Indianapolis, IN. pp 125-128.
  • Liu, S., M.D. Hall, C.A. Griffey, A.L. McKendry, J. Chen, and D.A. Van Sanford. 2008. Mapping QTL for scab resistance in the Virginia wheat cultivar Massey. In: Canty, S.M., E. Walton, A. Clark, D. Ellis, J. Mundell, and D.A. Van Sanford (Eds.), Proceedings of the National Fusarium Head Blight Forum; 2008 Dec 2-4; Indianapolis, IN. pp 176-177.
  • Liu, S., C. Griffey, A.L. McKendry, and M. Hall. 2008. Saturation mapping of scab resistance QTL in Ernie and application to marker-assisted breeding. In: Canty, S.M., E. Walton, A. Clark, D. Ellis, J. Mundell, and D.A. Van Sanford (Eds.), Proceedings of the National Fusarium Head Blight Forum; 2008 Dec 2-4; Indianapolis, IN. pp 180.
  • Liu, S., M.D. Hall, C.A. Griffey, A.L. McKendry 2009. Meta-analysis of QTL Associated with Fusarium Head Blight Resistance in Wheat. Crop Sci. 49:1955-1968
  • Rottinghaus, G.E. B.K. Tacke, T.J. Evans, M.S. Monstrom, L.E. Sweets, and A.L. McKendry. 2009. Fusarium Mycotoxin Concentrations in Straw, Chaff and Grain of Soft Red Winter Wheat Expressing a Range of resistance to Fusarium Head Blight. In: Canty, S.M. A. Clark, J. Mundell, E. Walton, D. Ellis, and D. Van Sanford (Eds.) Proceedings of the National Fusarium Head Blight Forum 2009 Dec 7-9; Orlando, FL. Lexington KY: University of Kentucky. pp.10.
  • Liu, S. C.A. Griffey, A.L. McKendry, M.D. Hall. 2009. Saturation Mapping of Scab Resistance QTL in Ernie and Identification of Diagnostic Markers for Breeding Scab Resistance. In: Canty, S.M. A. Clark, J. Mundell, E. Walton, D. Ellis, and D. Van Sanford (Eds.) Proceedings of the National Fusarium Head Blight Forum 2009 Dec 7-9; Orlando, FL. Lexington KY: University of Kentucky. pp.133
  • Liu, S., M.D. Hall, C.A. Griffey, A.L. McKendry, J. Chen, W.S. Brooks, G. Brown-Guedira, and D. Van Sanford. 2009. Saturation Mapping QTL for Scab Resistance in the Virginia Wheat Cultivar Massey. In: Canty, S.M. A. Clark, J. Mundell, E. Walton, D. Ellis, and D. Van Sanford (Eds.) Proceedings of the National Fusarium Head Blight Forum 2009 Dec 7-9; Orlando, FL. Lexington KY: University of Kentucky. pp.134.
  • Liu, S., M.D. Hall, C.A. Griffey, A.L. McKendry, J. Chen, G. Brown-Guedira, J.P. Murphy, and D. Van Sanford. 2009. Identification of Diagnostic Markers for FHB Resistance in Two US wheat Cultivars and their Application to Marker-assisted Breeding. 2009 American Society of Agronomy Meetings.
  • McKendry, A.L., D.N. Tague, J.K. Solomon, B.J. Craughwell. 2009. Missouri Winter Wheat Performance Tests. Missouri Agricultural Experiment Station, College of Agriculture, Food and Natural Resources. July 2009. Published at http://agebb.missouri.edu/cropperf/.


Progress 01/01/07 to 12/31/07

Outputs
OUTPUTS: The major objective of the Wheat Breeding Project is to develop varieties of soft red winter wheat and/or enhanced germplasm with improved grain yield, quality and disease resistance. Research within the breeding program continues to focus on the identification and genetic analyses of new sources of resistance to Fusarium head blight (FHB) caused by the fungus Fusarium graminearum Schwabe (teleomorph Gibberella zeae (Schwein.). In 2007 work continued on the genetic analyses of components of FHB resistance in University of Missouri releases Ernie and Truman. In 2006, four QTL regions were identified in Ernie associated with type II resistance to FHB. These regions, on chromosomes 2B, 3BSc, 4BL and 5AS together explained 43.3% of the phenotypic variation in resistance. In 2007 a further mapping study detected three QTL regions on 3BSc, 4BL and 5AS that were consistently associated with reduced deoxynivalenol (DON) accumulation in the grain and kernel quality retention under disease pressure, measured as percentage Fusarium damaged kernels (FDK). These QTL were coincidental with those for type II resistance in Ernie. The broad-based resistance in Truman which includes types I and II resistance, low DON and kernel quality retention under disease pressure will be studied in a collaborative project across 4 states including Kentucky (University of Kentucky), Indiana (Purdue University), Ohio (The Ohio State University) and Missouri (University of Missouri) over the next two years. Collaborative field phenotyping will be done across all locations while genotyping will be done at Missouri. In 2007, a set of 250 recombinant inbred lines (RILs) from the cross Truman/MO 94-317 was increased and screened for type II resistance in a replicated greenhouse study at Missouri. Diallel analyses of this and 19 other genotypes indicated that Truman not only has among the best sources of FHB resistance nationally, it also has excellent general and specific combining ability for type II resistance. In 2007 we also began a collaborative effort with Floyd Dowell at the USDA-ARS, Grain Marketing and Production Research Center, Engineering Research Unit, Manhattan, KS and Dr. Stephen Baenziger, Wheat Breeder at the University of Nebraska-Lincoln. Dr. Dowell is attempting to use an automated single-kernel near-infrared (SKNIR) system to separate seeds in heterogeneous mixtures based on specific quality attributes such as protein content, hardness, vitreousness, color, and degree of FHB damage. This technology has the potential to increase the efficiency of identifying FHB resistant segregates in bulk populations from resistant/susceptible crosses. Within the breeding program per se, MO 011126, entered in the Uniform Eastern Soft Red Winter Wheat Nursery is being considered for release. It is a very high yielding, high test weight mid-season variety that has exceptional milling and baking quality and an excellent disease package. A decision on the release of this line will be made in 2008. PARTICIPANTS: Principle Investigator: Dr. Anne L. McKendry Wheat Breeding Staff at the University of Missouri: David Tague, Research Specialist; Brendan Craughwell, Research Specialist; Julie Solomon, Senior Research Laboratory Technician. Students: Zewdie Abate; Doctoral Student Collaborators outside the University of Missouri: Stephen Baenziger, University of Nebraska Floyd Dowell, USDA-ARS, Grain Marketing and Production Research Center Manhattan Kansas Dr. Shuyu Liu, Research Scientist Virginia Tech University Dr. Ed Souza, USDA-ARS Soft Wheat Quality Laboratory, Wooster, OH Dr. David Van Sanford, University of Kentucky Dr. Herb Ohm, Purdue University Dr. Clay Sneller, The Ohio State University U.S. Wheat and Barley Scab Initiative has funded much of the Fusarium head blight research through USDA-ARS grants. TARGET AUDIENCES: Target groups include scientists (wheat breeders, pathologists, molecular geneticists) who are reached through peer-reviewed literature and growers who are reached through variety release and publication of the Missouri Winter Wheat Performance Test bulletins.

Impacts
This research will lead to the development and release to Missouri growers, of adapted varieties of soft red winter wheat that have higher yield potential, excellent test weight, good end use quality and resistance to pathogens relevant in the Missouri wheat growing areas. It will also lead to increased breeding efficiency that should accelerate the development of these improved wheat varieties thus enhancing economic return for wheat growers both in Missouri and in the surrounding states. In 2007, we continued to see broad adoption of Missouri's Fusarium head blight resistant varieties Truman and Bess across the soft red winter wheat region. Truman exemplifies this adoption. In 2007 it was grown from south-eastern Kansas to New York in the north east. Research within the breeding program has led to the identification of QTL associated with components of FHB resistance in Ernie including reduced disease severity, low deoxynivalenol (DON) contamination of the resulting grain and fewer Fusarium damaged kernels. Information on these QTL has been disseminated to interested breeders both through papers/posters at the U.S. National Fusarium Head Blight Forum and through the peer-reviewed literature. Once validated, markers associated with these QTL will be available for use in breeding programs using marker-assisted-selection to enhance the efficiency of incorporating FHB resistance genes into their varieties. Where Dr. Dowell's efforts to automate the separation of Fusarium damaged kernels proves to have heritable results, it should further increase the efficiency of identifying FHB resistant segregates in bulk populations from resistant/susceptible crosses. Each of these research projects will increase breeding efficiency thereby accelerating the development of FHB resistant varieties for Missouri and the surrounding areas. The release of these varieties will strengthen the wheat-based economy of the state and where DON levels in the harvested grain are reduced, they will improve the safety of the wheat-based food supply.

Publications

  • McKendry, A.L., D.N. Tague, R.L. Wright, J.A. Tremain. 2007. Registration of Bess Wheat. Crop Science Journal of Plant Registrations 1:21-23
  • S. Liu, Z. A. Abate, H. Lu, T. Musket, G.L. Davis, and A.L. McKendry. 2007. QTL associated with Fusarium head blight resistance in the soft red winter wheat Ernie. Theor. Appl. Genet. 115:417-427
  • Abate, Z. A., S.Liu, and A.L. McKendry. 2007. Quantitative Trait Loci Associated with deoxynivalenol Content and Kern Quality in the Soft Red Winter Wheat, 'Ernie'. Crop Science. In Press.
  • McKendry, A.L. 2007. Breeding for FHB resistance in winter wheat: What's ahead? In: Canty, S.M., Clark, A., Ellis, D., and VanSanford D. (Eds)Proceedings of the National Fusarium Head Blight Forum. 2-4 December 2007; Westin Crown Center, Kansas City, MO. 2-4 December pp. 204
  • Liu, S., C.A. Griffey, A.L. McKendry, and M.D. Hall. 2007. Meta-analyses of QTL associated with Fusarium head blight resistance. In: Canty, S.M., Clark, A., Ellis, D., and VanSanford D. (Eds)Proceedings of the National Fusarium Head Blight Forum. 2-4 December 2007; Westin Crown Center, Kansas City, MO. 2-4 December pp. 195-200.
  • McKendry, A.L. D.N. Tague, J.K. Solomon. 2007. 2007 Missouri Winter Wheat Performance Tests. Special Report 569. Missouri Agricultural Experiment Station, College of Agriculture, Food, and Natural Resources. University of Missouri. July 2007. 28 pages.