Source: MISSISSIPPI STATE UNIV submitted to
IN VITRO PROPAGATION OF ELITE PECAN (CARYA ILLINOINENSIS (WANGENH.) K. KOCH) CULTIVARS
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
TERMINATED
Funding Source
Reporting Frequency
Annual
Accession No.
0209809
Grant No.
2006-38814-17442
Project No.
MIS-121030
Proposal No.
2006-02858
Multistate No.
(N/A)
Program Code
EQ
Project Start Date
Sep 1, 2006
Project End Date
Aug 31, 2010
Grant Year
(N/A)
Project Director
Matta, F.
Recipient Organization
MISSISSIPPI STATE UNIV
(N/A)
MISSISSIPPI STATE,MS 39762
Performing Department
PLANT & SOIL SCIENCES
Non Technical Summary
Propagation of pecan is mainly done by budding or grafting on rootstocks. However, budding or grafting involves considerable time and expense often with only moderate success. The technology of micropropagation (regeneration of plants through tissue culture) or in vitro propagation of plants has been used successfully to produce large scale generation of selected clones within a short time for several forest trees that were otherwise difficult to propagate. Regeneration of true-type elite plants will potentially be of great use to the Arkansas and Mississippi pecan industry. This project examines the effectiveness of micropropagation of elite pecan cultivars through direct regeneration (bud culture) and in vitro regeneration of pecan plants through organogenesis or somatic embryogenesis. The purpose of this study is to produce elite pecan cultivars trees via tissue culture and the production of uniform rootstocks.
Animal Health Component
40%
Research Effort Categories
Basic
40%
Applied
40%
Developmental
20%
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2051211103030%
2051211104030%
2051211106040%
Goals / Objectives
Micropropagation of elite pecan cultivars through direct regeneration (bud culture). In vitro regeneration of pecan plants through organogenesis or somatic embryogenesis using young leaf, petiole and stem. Evaluation of micropropagated pecan plants. Distribution of uniform pecan seedlings to selected farmers, researchers, and nursery producers. Training undergraduate students in micropropagation techniques. Establishment of strong collaboration with multi-institutions. Strengthening of research capacity at UAPB.
Project Methods
In this proposal, we propose to micropropagate commercially important pecan cultivars through direct regeneration from buds --i.e. bud culture. Attempts will also be made to produce plants through organogenesis or somatic embryogenesis from leaves, petioles and stems in order to maintain genetic uniformity. Regenerated plants will be evaluated for genetic fidelity by AFLP before being supplied to the nurseries for distribution for the growers. Regenerated plants will also be tested by the collaborating PIs in the nurseries and field to assess the performance of regenerated plants at different locations.

Progress 09/01/06 to 08/31/10

Outputs
OUTPUTS: Pecan Plantlets produced via tissue culture have been established in one gallon containers. The plants were initially evaluated at University of Arkansas, Pine Bluff. Young pecan trees will be transplanted on the main campus at Mississippi State University and evaluated for over all productivity. Additional tissue culture protocols to reduce explant contamination and increase the member of explants are being evaluated. PARTICIPANTS: Nothing significant to report during this reporting period. TARGET AUDIENCES: Nothing significant to report during this reporting period. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
Pecan nurserymen will enable to provide growers with disease free plants that are true to cultivar. Also, clonal rootstocks can be developed. Growers will benefit from the uniformity of production of desired cultivars and also uniformity of clonal rootstocks to produce uniform scions of grafted cultivars. Furthermore, rapid propagation of numerous plants will be responsible.

Publications

  • No publications reported this period


Progress 09/01/07 to 08/31/08

Outputs
OUTPUTS: Multiple pecan shoots were successfully induced on modified Woody Plant Medium (WPM) containing 13.32 micromole BAP, from axillary buds of mature trees such as Desirable, Stuart, Sumner, and Cape Fear. The multiple shoots were separated and successfully rooted in liquid WPM containing 49.20 micromole indole-2-butyric acid. The pecan plantlets were initially transferred to peat pellets and subsequently to the greenhouse, then potted in one gallon containers. The pecan plants will be planted in different locations in Arkansas, Mississippi, and New Mexico in 2010. PARTICIPANTS: Nothing significant to report during this reporting period. TARGET AUDIENCES: Nothing significant to report during this reporting period. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
Pecan nurserymen will e able to provide growers with disease free plants that are true to cultivar. Also, clonal rootstocks can be developed. Growers will benefit from the uniformity of production of desired cultivars and also uniformity of clonal rootstocks to produce uniform scions of grafted cultivars. Furthermore, rapid propagation of numerous plants will be responsible.

Publications

  • No publications reported this period


Progress 09/01/06 to 08/31/07

Outputs
OUTPUTS: Pecan trees were propagated via tissue culture by using embryo rescue and mature buds as explants. Propagation was successful regardless of explants used, however, a technique to reduce contamination of explants was developed to increase survival rate. Plants produced roots and shoots and were transplanted to 15 centimeter pots and evaluated under greenhouse conditions. Plants propagated from buds are more desirable since they will be true to cultivar. Plants propagated via embryo rescue were highly variable in growth characteristics. PARTICIPANTS: Nothing significant to report during this reporting period. TARGET AUDIENCES: Nothing significant to report during this reporting period. PROJECT MODIFICATIONS: Nothing significant to report during this reporting period.

Impacts
Pecan nurserymen will be able to provide growers with disease free plants that are true to cultivar. Also, clonal rootstocks can be developed. Growers will benefit from the uniformity of production of desired cultivars and also uniformity of clonal rootstocks to produce uniform scions of grafted cultivars. Furthermore, rapid propagation of numerous plants will be possible.

Publications

  • No publications reported this period