Source: UNIVERSITY OF TENNESSEE submitted to
SOYBEAN BREEDING
Sponsoring Institution
National Institute of Food and Agriculture
Project Status
TERMINATED
Funding Source
Reporting Frequency
Annual
Accession No.
0181757
Grant No.
(N/A)
Project No.
TEN00201
Proposal No.
(N/A)
Multistate No.
(N/A)
Program Code
(N/A)
Project Start Date
Oct 1, 1999
Project End Date
Sep 30, 2004
Grant Year
(N/A)
Project Director
Pantalone, V. R.
Recipient Organization
UNIVERSITY OF TENNESSEE
1331 CIRCLE PARK DR
KNOXVILLE,TN 37916-3801
Performing Department
PLANT SCIENCES & LANDSCAPE SYSTEMS
Non Technical Summary
(N/A)
Animal Health Component
80%
Research Effort Categories
Basic
20%
Applied
80%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
2011820108015%
2021820108010%
2031820108010%
2041820108020%
2041820102015%
2121820108015%
2131820108015%
Goals / Objectives
1)Develop improved cultivars with enhanced productivity or added-value that are adapted to the climatic/edaphic conditions and cultural practices in Tennessee and surrounding states, 2)Develop high yielding germplasm or cultivars with modified oil quality, enhanced protein concentration, or other value-added traits. 3)Obtain new information on soybean genetics and breeding methodology, and 4)explore and adapt new technologies in order to improve the efficiency of soybean breeding.
Project Methods
1a)Hybridization of diverse parental stocks. b)Early generations will be advanced via the multiple-seed descent method in winter nursery facilities. c)Later genertions will be handled via the following breedng methods: pedigree, bulk, and recurrent selection. d)Lines will be evaluated in replicated yield tests under different environmental/cultural conditions. e)Germplasm lines and superior breeding lines will be increased and released for breeding purposes and commercial production, respectively. 2a)Modifications of breeding techniques will be explored through statistical measures in order to make the breeding process more effective. b)Appropriate crosses will be made among parental stocks in order to study the inheritance of various qualitative traits deemed to be important for maximum production or for added-value. c)Biotic/abiotic factors of the environment will be enhanced or diminished in order to determine which factors provide the greatest opportunity for genetic improvement. 3a)Explore new technologies which will reduce the time, labor, and human errors involved in various aspects of the breeding program. b)Investigate ways to adapt new advances in biotechnology for the improvement of desirable attributes.

Progress 10/01/99 to 09/30/04

Outputs
The Tennessee Agricultural Experiment Station's new high yielding conventional soybean cultivar 5002T was adopted by breeders in 2004 to become the new high yield check cultivar, replacing Manokin, in the USDA Southern Uniform testing program. Cultivar 5002T was registered by the Crop Science Society of America as CV-466 and seed is currently available in the active collection of the Germplasm Resource Information Network as PI 634193 and is being readily made available to breeders for research and applied cultivar improvement programs. A total of 5,744 yield test plots were conducted at the Milan, West Tennessee, Highland Rim, and Knoxville Agricultural Experiment Stations by the University of Tennessee (UT) Soybean Breeding and Genetics Program in 2004. A total of 18 advanced UT lines were entered in the USDA Southern Regional Preliminary and Uniform Tests in 2004. Two new cultivars (5002T and 5601T) and eight new UT lines were entered in the 2004 Tennessee State Variety Test. Our program currently is evaluating the potential of several lines as possible new cultivars or germplasm which may become important materials for breeders to utilize for genetics gains in seed quality and yield performance. Approximately 14,218 pure-line progeny rows were subjected to visual selection for agronomic traits such as plant productivity, maturity, and lodging resistance in TN during 2004. The top 10-15% of lines were selected. We developed 54 new cross combinations in 2004. Our primary targets were high yield, resistance to soybean cyst nematode, resistance to sudden death syndrome, increased oleic acid, reduced saturated fat and reduced trans fat. Other targets included higher protein and low phytate. Hundreds of UT experimental lines were screened for resistance to soybean cyst nematode at the USDA-ARS nematology laboratory at the West Tennessee Experiment Station in Jackson, TN. Genomic regions revealing quantitative trait loci for seed yield, plant height, seed protein and oil concentration, fatty acid composition, amino acid composition, 11S and 7S fractions of the seed protein, seed size, and plant maturity were detected from mapping populations in our program during 2004.

Impacts
The new Tennessee Agricultural Experiment Station soybean cultivar 5601T was approved in 2003 as the new USDA yield check cultivar for its maturity class. This represents an achieved milestone because 5601T soybean will serve as the gauge of success towards which other breeding programs will strive to match or beat for high yield performance. The discovery of genomic regions governing the amino acids cysteine and methione by our program in 2003 has important implications for improving soybean protein quality for human and livestock nutrition. The new Tennessee Agricultural Experiment Station soybean variety 5002T exhibited excellent resistance to the devastating disease, soybean sudden death syndrome (SDS), in field trials in 2003 and in previous years.

Publications

  • Hyten, D.L, V.R. Pantalone, C.E. Sams, A.M. Saxton, D. Landau-Ellis, T.R. Stefaniak, M.E. Schmidt. 2004. Seed Quality QTL in a Prominent Soybean Population. Theor. Appl. Genet. 109:552-561.
  • Pantalone, V.R., F.L. Allen, and D. Landau-Ellis. 2004. Registration of 5002T soybean. Crop Sci. 44:1483-1484.
  • Pantalone, V.R., D.R. Walker, R.E. Dewey, and I. Rajcan. 2004. DNA Marker-Assisted Selection for Improvement of Soybean Oil Concentration and Quality, In R.F. Wilson, H.T. Stalker, and R.C. Brummer (eds.) Legume Crop Genomics pp. 283-311.
  • Panthee, D.R., V.R. Pantalone, C.E. Sams, A.M. Saxton, D.R. West, and W.E. Rayford,. 2004. Genomic Regions Governing Soybean Seed Nitrogen Accumulation. J. Am. Oil Chem. Soc. 81: 77-81.
  • Spencer, M.M., D. Landau-Ellis, E.J. Meyer, and V.R. Pantalone. 2004. Molecular Markers Associated with Linolenic Acid Content in Soybean. J. Am. Oil Chem. Soc. 81: 559-562.


Progress 01/01/03 to 12/31/03

Outputs
Adoption of Newly Released Conventional Soybean Cultivar: 5601T The Tennessee Agricultural Experiment Station recently released a new high yielding maturity group 5.6 conventional soybean cultivar, 5601T. Farmers have begun to adopt this cultivar, with approximately 12,141 hectares produced in Tennessee and nearby states during 2003. On-farm yields were excellent, typically exceeding 3,500 kg ha-1 during 2003. Yield Testing and New Cultivar Development: A total of 7,371 yield test plots were conducted at the Milan, West Tennessee, Ames Plantation, Highland Rim, and Knoxville Agricultural Experiment Stations by the University of Tennessee (UT) Soybean Breeding and Genetics Program in 2003. A total of 13 advanced UT lines were entered in the USDA Southern Regional Preliminary and Uniform Tests in 2003. Two new cultivars (5002T and 5601T) and nine new UT lines were entered in the 2003 Tennessee State Variety Test. Our program currently is evaluating the potential of several lines as possible new cultivars or germplasm which may become important materials for breeders to utilize in their soybean improvement programs. Population Development: Approximately 6,422 pure-line progeny rows were subjected to visual selection for agronomic traits such as plant productivity, maturity, and lodging resistance in TN during 2003. The top 15% of rows were selected to carry forward in next year. We developed 34 new cross combinations in 2003. Our primary targets were high yield, resistance to soybean cyst nematode, increased oleic acid, reduced saturated fat and reduced trans fat. Other targets included higher protein and low phytate. Hundreds of UT experimental lines were screened for resistance to one or more races of soybean cyst nematode at the USDA-ARS nematology laboratory at the West Tennessee Experiment Station in Jackson, TN. Molecular Genetic Research Genomic regions revealing quantitative trait loci for yield, plant height, seed protein and oil concentration, fatty acid composition, amino acid composition, seed size, and maturity were detected from mapping populations in our program. Continued molecular genetics research will further help to develop applied, molecular marker-assisted breeding approaches.

Impacts
The new Tennessee Agricultural Experiment Station soybean cultivar 5601T was approved in 2003 as the new USDA yield check cultivar for its maturity class. This represents an achieved milestone because 5601T soybean will serve as the gauge of success towards which other breeding programs will strive to match or beat for high yield performance. The discovery of genomic regions governing the amino acids cysteine and methione by our program in 2003 has important implications for improving soybean protein quality for human and livestock nutrition. The new Tennessee Agricultural Experiment Station soybean variety 5002T exhibited excellent resistance to the devastating disease, soybean sudden death syndrome (SDS), in field trials in 2003 and in previous years.

Publications

  • Pantalone, V.R., F.L. Allen, and D. Landau-Ellis. 2003. Registration of 5601T soybean. Crop Sci. 43:1123-1124.
  • Pantalone, V.R., F.L. Allen, and D. Landau-Ellis. 2003. Registration of TN93-99 soybean germplasm. Crop Sci. 43:1137.
  • Chapman, A., V.R. Pantalone, A. Ustun, F.L. Allen, D. Landau-Ellis, R.N. Trigiano, and P.M. Gresshoff. 2003. Quantitative trait loci for agronomic and seed quality traits in an F2 and F4:6 soybean population. Euphytica 129:387-393.
  • Cherrak, C.M, V.R. Pantalone, E.J. Meyer, D.L. Ellis, S.L. Melton, D.R. West, and J.R. Mount. 2003. Low palmitic, low linolenic soybean development. J. Am Oil Chem. Soc. 6:539-543.
  • Spencer, M.M., V.R. Pantalone, E.J. Meyer, D. Landau-Ellis, and D.L. Hyten, Jr. 2003. Mapping the Fas locus controlling stearic acid content in soybean. Theor. Appl. Genet. 106:615-619.


Progress 01/01/02 to 12/31/02

Outputs
Release of New Conventional Soybean Cultivar, 5002T The Tennessee Agricultural Experiment Station has released a new high yielding maturity group (MG) 5.0 conventional soybean cultivar, 5002T. 5002T is most similar in appearance to the cultivar Manokin. 5002T has white flowers, tawny pubescence, a determinate growth habit, and is tolerant or resistant to stem canker, frogeye leaf spot and sudden death syndrome, however it is susceptible to soybean cyst nematode. Based on data averaged over the 1998, 1999, 2000 and 2001 Uniform tests, 5002T produced 3151 kg ha-1 seed yield, with 200 g kg-1 seed oil, 414 g kg-1 seed protein, and 13.8 g 100 seeds-1, compared with USDA check Manokin at 2856 kg ha-1 seed yield, 195 g kg-1 seed oil, 415 g kg-1 seed protein, and 12.3 g 100 seeds-1. Yield Testing and New Cultivar Development: A total of 5,248 yield test plots were conducted at the Milan, West Tennessee, Ames Plantation, Highland Rim, and Knoxville Agricultural Experiment Stations by the University of Tennessee (UT) Soybean Breeding and Genetics Program in 2002. A total of 20 advanced UT lines were entered in the Southern Regional Preliminary and Uniform Tests in 2002. Two new cultivars (5002T and 5601T) and three UT lines were entered in the 2002 Tennessee State Variety Test. Our program currently is evaluating the potential of several lines as possible new cultivars or germplasm which may become important materials for breeders to utilize in their soybean improvement programs. Population Development: Approximately 5,969 pure-line progeny rows and 219,520 newly derived single-plants were subjected to visual selection for agronomic traits such as plant productivity, maturity, and lodging resistance at Knoxville, TN in 2002. The top 15% of rows were selected to carry forward in 2003. We developed 45 new cross combinations in 2002. Our primary targets were high yield, resistance to soybean cyst nematode, increased oleic acid, reduced saturated fat and reduced trans fat. Other targets included low lipoxygenase, higher protein, and low phytate. Hundreds of UT experimental lines were screened for resistance to one or more races of soybean cyst nematode at the USDA,ARS nematology laboratory at the West Tennessee Experiment Station in Jackson, TN. Molecular Research Quantitative trait loci for yield, plant height, seed protein and oil concentration, fatty acid composition, amino acid composition, seed size, and maturity were discovered from mapping populations in our program. Continued molecular genetics research will further help to develop applied, molecular marker-assisted breeding approaches.

Impacts
(N/A)

Publications

  • Pantalone, V.R., R.F. Wilson, W.P. Novitzky, and J.W. Burton. 2002. Genetic regulation of elevated stearic acid concentration in soybean oil. Journal of the American Oil Chemists? Society 79:549-553.
  • Hyten, David L., Jr. 2002. QTL Mapping and Identification of GxE Interactions of Agronomic and Seed Quality Traits in Soybean. M.S. Thesis. University of Tennessee.
  • Stokes, Yamika N. 2002. Quantitative Trait Loci and Heritability Analysis of TN93-99 x PI 416937 F6 Derived Soybean Progeny. M.S. Thesis. University of Tennessee.


Progress 01/01/01 to 12/31/01

Outputs
Release of New Conventional Soybean Variety, 5601T The Tennessee Agricultural Experiment Station has released a new high yielding maturity group (MG) V conventional soybean variety, 5601T. Based on data averaged over the 1998, 1999, and 2000 Uniform tests, 5601T produced 3144 kg ha-1 seed yield, with 192 g kg-1 seed oil, 421 g kg-1 seed protein, and 12.7 g 100 seeds-1, compared with USDA check 'Hutcheson' at 2882 kg ha-1 seed yield, 203 g kg-1 seed oil, 410 g kg-1 seed protein, and 13.2 g 100 seeds-1. Release of New Conventional Soybean Germplasm, TN93-99 The Tennessee Agricultural Experiment Station has released a new high yielding MG V conventional soybean germplasm, TN93-99. Based on the data averaged over the 1996, 1997, 1998, and 2000 Uniform tests, TN93-99 produced 3192 kg ha-1 seed yield, with 208 g kg-1 seed oil, 416 g kg-1 seed protein, and 13.3 g 100 seeds-1, compared with 'Hutcheson' at 2990 kg ha-1 seed yield, 208 g kg-1 seed oil, 410 g kg-1 seed protein, and 13.5 g 100 seeds-1. Yield Testing and Potential New Varieties: A total of 4,542 yield test plots were conducted at the Milan, West Tennessee, Ames Plantation, Highland Rim, and Knoxville locations of the University of Tennessee (UT) Agricultural Experiment Station by the UT Soybean Breeding and Genetics Program in 2001. A total of 23 advanced UT lines were entered in the Southern Regional Preliminary and Uniform Tests in 2001. Three UT lines were entered in the 2001 Tennessee State Variety Test. Our program currently is evaluating the potential of several lines as possible new varieties. A new line, TN96-68, tied for #1 (along with 5601T) for seed yield (3360 kg ha-1), averaged across seven environments, among all conventional MG V entries in the 2001 Tennessee State Variety Test. TN96-68 also ranked #1 for resistance to sudden death syndrome (SDS) in the 2000 Southern Illinois SDS field trials, and may become an important line for breeders to utilize in developing resistant varieties. Population Development: Approximately 15,066 pure-line progeny rows and 42,240 newly derived single-plants were subjected to visual selection for agronomic traits such as plant productivity, maturity, and lodging resistance at Knoxville, TN in 2001. The top 10% of rows were selected to carry forward in 2002. We developed 49 new cross combinations in 2001. Our primary targets were high yield, resistance to SCN Races 2,3,5, or 14, and low saturated, low linolenic fatty acid. Other targets included increased oleic acid, low lipoxygenase, higher protein, and low phytate. Hundreds of UT experimental lines were screened for resistance to one or more races of soybean cyst nematode (SCN) at the USDA,ARS nematology laboratory at the West Tennessee Experiment Station in Jackson, TN. Molecular Research Quantitative trait loci (QTL) for yield, plant height, seed protein and oil content, fatty acids, seed size, and maturity were discovered from mapping populations in our program. Continued molecular genetics research will further help to develop applied, molecular marker-assisted breeding approaches.

Impacts
(N/A)

Publications

  • G.J. Rebetzke, V.R. Pantalone, J.W. Burton, T.E. Carter, Jr., and R.F. Wilson. 2001. Genetic Background and Environment Influence Palmitate Content of Soybean Seed Oil. Crop Sci. 41:1731-1736.
  • Cherrak, C.M. 2001. Breeding Low Palmitic Low Linolenic Soybeans. M.S. Thesis. University of Tennessee, Knoxville, TN.


Progress 01/01/00 to 12/31/00

Outputs
Yield Testing and Potential New Varieties: A total of 5,044 yield test plots were conducted at the Milan, West Tennessee, Ames Plantation, Highland Rim, and Knoxville locations of the University of Tennessee (UT) Agricultural Experiment Station by the UT Soybean Breeding and Genetics Program in 2000. A total of 25 advanced UT lines were entered in the Southern Regional Preliminary and Uniform Tests in 2000. Six UT lines were entered in the Tennessee State Variety Test. Our program currently is evaluating the potential of several lines as possible new varieties within the next year. We entered lines TN93-87, TN93-99, TN94-213, and TN93-142-17 in several state variety tests in the region. A new line TN96-58, ranked #1 for yield, averaged across six environments, among all 38 conventional maturity group (MG) V entries in the 2000 Tennessee State Variety Test. TN96-58 ranked #1 for yield in seven different Tennessee field trials over the past five years, averaging 3192 kg/ha, representing significantly higher yield than the widely-grown variety 'Hutcheson'. Phenotypic Selection and Nematode Resistance Screening: Approximately 4,622 pure-line progeny rows and 74,000 newly derived single-plants were subjected to visual selection for agronomic traits such as plant productivity, maturity, and lodging resistance at Knoxville, TN in 2000. The top 10% of rows and single-plants were selected to carry forward in 2001. Hundreds of UT experimental lines were screened for resistance to one or more races of soybean cyst nematode (SCN) at the USDA,ARS nematology laboratory at the West Tennessee Experiment Station in Jackson, TN. New Cross Combinations: We developed 50 new bi-parental hybridization combinations in 2000. Our primary targets were high yield, resistance to SCN Races 2,3,5, or 14, and low saturated, low linolenic fatty acid. Other targets included increased oleic acid, low lipoxygenase, higher protein, and low phytate. Genetic Research: Quantitative trait loci (QTL) for yield, plant height, seed protein and oil concentration, seed size, and maturity were discovered in a population from a cross between the soybean varieties 'Essex' and 'Williams.' For the F2 population, significant additive QTL were SATT540 (maturity, r2 = 0.11; height, r2 = 0.04), SATT52 (yield, r2 = 0.02; height, r2 = 0.19), SATT239 (yield, r2 =0.13; seed size, r2 = 0.20), and SATT373 (yield, r2 = 0.02; oil, r2 = 0.02; seed size, r2 = 0.23; height, r2 = 0.28). In the F4:6 generation, averaged over both environments, significant additive QTL were identified: SATT52 (yield, r2 = 1.8%), SATT251 (protein, r2 = 2.6%), SATT353 (seed size, r2 = 2.5%), SATT14 (seed size, r2 = 11.3%), and SATT239 (height, r2 = 2.6%). Continued molecular genetics research with our Essex x Williams population may further help to develop applied, molecular marker-assisted approaches for soybean breeders.

Impacts
(N/A)

Publications

  • Allen, F.L., L.D. Young, M.A. Newman, D.Landau-Ellis, and V.R. Pantalone. 2000. Registration of `TN 5-95' Soybean. Crop Science 40:575-576.
  • Chapman, A. 2000. QTL and Heritability Analysis of Essex x Williams F2 and F4:6 Soybean Progenies. Master of Science Thesis. University of Tennessee.
  • Chapman, A., V.R. Pantalone, D. Landau-Ellis, F.L. Allen, P.M. Gresshoff, and R.N. Trigiano. 2000. Quantitative Trait Loci in an Essex x Williams Soybean Population. Agronomy Abstracts.


Progress 01/01/99 to 12/31/99

Outputs
230 maturity group (MG) IV and 520 MG V soybean genotypes were evaluated in 20 different replicated field experiments at University of Tennessee Agricultural Experiment Stations in 1999. One of our newest advanced lines, TN96-58, which ranked #1 for seed yield in the 1998 Southern Uniform Preliminary test also ranked #1 in the 1999 Southern Uniform test at the Knoxville, TN location, producing 3369 kg/ha seed yield. Some field experiments included new high protein and modified fatty acid genotypes. New genotypes with low palmitic and low linolenic fatty acids were identified in a preliminary trial producing seed yields equivalent to that of the check. Approximately 10,000 progeny rows were screened in the field and selected lines were identified for yield testing in 2000.

Impacts
(N/A)

Publications

  • No publications reported this period