Source: TEXAS A&M UNIVERSITY submitted to
PASTEURELLA HAEMOLYTICA: THE EFFECTS OF ANTIBIOTICS ON THE PRODUCTION OF CONSTITUTIVE ENZYMES
Sponsoring Institution
State Agricultural Experiment Station
Project Status
TERMINATED
Funding Source
Reporting Frequency
Annual
Accession No.
0179945
Grant No.
(N/A)
Project No.
TEX08656
Proposal No.
(N/A)
Multistate No.
(N/A)
Program Code
(N/A)
Project Start Date
Oct 1, 1998
Project End Date
Mar 31, 2006
Grant Year
(N/A)
Project Director
Scanlan, C. M.
Recipient Organization
TEXAS A&M UNIVERSITY
750 AGRONOMY RD STE 2701
COLLEGE STATION,TX 77843-0001
Performing Department
VETERINARY PATHOBIOLOGY
Non Technical Summary
Pasturellosis of cattle causes disease and economic loss. The purpose of this investigation is to determine the effects of selected antibiotics on the enzymatic activities and protective immunogens produced by two strains pf Pasteurella haemolytica with demonstrated antibiotic resistance that were isolated from the bovine respiratory disease complex.
Animal Health Component
50%
Research Effort Categories
Basic
50%
Applied
50%
Developmental
(N/A)
Classification

Knowledge Area (KA)Subject of Investigation (SOI)Field of Science (FOS)Percent
31133991103100%
Knowledge Area
311 - Animal Diseases;

Subject Of Investigation
3399 - Beef cattle, general/other;

Field Of Science
1103 - Other microbiology;
Goals / Objectives
The objective of these investigations are to determine the effects of selected antibiotics on the enzymatic activities and protective immunogens produced by two strains of Pasteurella haemolytica with demonstrated antibiotic resistance that were isolated from the bovine respiratory disease complex.
Project Methods
The two P. haemolytica strains will be cultured on Mueller-Hinton agar supplemented with 10 percent fetal calf serum (MHSA) and on MHSA containing the maximum concentrations of cephalothin, penicillin, streptomycin and tetracycline that permitted the growth of the organisms. The preformed enzyme levels of the two P. haemolytica strains will be determined using a commercial semiquantitative enzyme system. The results of these studies will be used to evaluate the expression of selected protective immunogens, such as a capsule and leukotoxin, by the two Pl haemolytica strains.

Progress 10/01/98 to 03/31/06

Outputs
The two bovine strains of pathogenic Pasteurella haemolytica (now classified as Mannheimia haemolytica) did not exhibit differences in their enzymatic activities or protective immunogens during these investigations. The antimicrobial susceptibility patterns to 24 antibacterial drugs and antibiotics varied marked as the organism were repeatedly subcultured on aerobic blood agar plates. This was documented to result from the loss of plasmid-mediated R-factors. Also, the study demonstrated differences in antibiotic resistance genes on the chromosome and plasmids in each strain.

Impacts
These investigations confirmed several principles and concepts of previous investigators. However, our studies did not delinate new publisable data.

Publications

  • No publications reported this period


Progress 01/01/04 to 12/31/04

Outputs
Research is continuing in order to determine the effects of selected antibiotics on the enzymatic activities and protective immunogens produced by two strains of Pasteurella haemolytica with demonstrated antibiotic resistance that were isolated from the bovine respiratory disease complex

Impacts
To improve the quality of health of feedlot cattle

Publications

  • No publications reported this period


Progress 01/01/03 to 12/31/03

Outputs
Research is continuing in order to determine the effects of selected antibiotics on the enzymatic activities and protective immunogens produced by two strains of Pasteurella haemolytica with demonstrated antibiotic resistance that were isolated from the bovine respiratory disease complex

Impacts
To improve the quality of health of feedlot cattle

Publications

  • No publications reported this period


Progress 01/01/02 to 12/31/02

Outputs
Research is continuing to determine the effects of selected antibiotics on the enzymatic activities and protective immunogens produced by two strains of Pasteurella haemolytica with demonstrated antibiotic resistance that were isolated from the bovine respiratory disease complex

Impacts
To improve the quality of health of feedlot cattle

Publications

  • No publications reported this period


Progress 01/01/01 to 12/31/01

Outputs
Research is in progress to determine the effects of selected antibiotics on the enzymatic activities and protective immunogens produced by two strains of Pasteurella haemolytica with demonstrated antibiotic resistance that were isolated from the bovine respiratory disease complex.

Impacts
(N/A)

Publications

  • No publications reported this period


Progress 01/01/00 to 12/31/00

Outputs
Research is in progress to determine the effects of selected antibiotics on the enzymatic activities and protective immunogens produced by two strains of Pastruella haemolytica with demonstrated antibiotic resistance that were isolated from the bovine respiratory disease complex.

Impacts
(N/A)

Publications

  • No publications reported this period


Progress 01/01/99 to 12/31/99

Outputs
Research is in progress to determine the effects of selected antibiotics on the enzymatic activities and protective immunogens produced by two strains of Pasteurella haemolytica with demonstrated antibiotic resistance that were isolated from the bovine respiratory disease complex.

Impacts
(N/A)

Publications

  • No publications reported this period